Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16600 and 16601 are separated by 205 nucleotides16601 and 16602 are separated by 225 nucleotides16602 and 16603 are separated by 417 nucleotides16603 and 16604 are separated by 169 nucleotides mRNA_8232: 16600 - K12623 LSM4 U6 snRNA-associated Sm-like protein LSm4, at 727,305 to 728,231 16600 mRNA_8233: 16601 - K13113 UBL5, HUB1 ubiquitin-like protein 5, at 728,437 to 729,216 16601 mRNA_8234: 16602 - KOG3702 Nuclear polyadenylated RNA binding protein, at 729,442 to 731,463 16602 mRNA_8235: 16603 - Hypothetical Protein, at 731,881 to 732,842 16603 mRNA_8236: 16604 - Interpro Fungal protein of unknown function (DUF1770) 0, at 733,012 to 733,833 16604
Group Condition mRNA_8232 mRNA_8233 mRNA_8234 mRNA_8235 mRNA_8236
16600 16601 16602 16603 16604
facs on lipid accumulation Sorted No BODIPY Gate -0.6 N.D. N.D. -0.3 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.9 N.D. N.D. -0.3 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.8 N.D. N.D. +0.6 -0.4
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. N.D. -0.3 -0.1
ypd buoyancy 40 hrs High Buoyancy -0.3 N.D. N.D. -0.3 +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. N.D. -0.2 +0.3
auxotrophy YNB Glucose -0.1 N.D. N.D. +0.0 +0.2
growth on ricinoleic acid YNB Glucose -0.1 N.D. N.D. +0.3 +0.0
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. N.D. -0.3 +0.4
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. N.D. -0.2 +0.2
ypd buoyancy 40 hrs Low Buoyancy -0.2 N.D. N.D. +0.1 +0.4
growth on ypd YPD +0.7 N.D. N.D. -0.7 +0.4
auxotrophy Dropout Complete -0.2 N.D. N.D. +0.0 +0.6
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 N.D. N.D. +0.3 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 N.D. N.D. +0.4 +0.1
growth on oleic acid YNB Glucose +0.6 N.D. N.D. +0.2 -0.2
growth on oleic acid YNB Oleic Acid +0.7 N.D. N.D. +0.1 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. N.D. +0.3 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.7 N.D. N.D. +0.1 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +1.0 N.D. N.D. +0.2 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 N.D. N.D. +0.6 +0.5
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