Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16603 and 16604 are separated by 169 nucleotides16604 and 16605 are separated by 393 nucleotides16605 and 16606 are separated by 609 nucleotides16606 and 16607 are separated by 287 nucleotides mRNA_8235: 16603 - Hypothetical Protein, at 731,881 to 732,842 16603 mRNA_8236: 16604 - Interpro Fungal protein of unknown function (DUF1770) 0, at 733,012 to 733,833 16604 mRNA_8237: 16605 - K04345 PKA protein kinase A, at 734,227 to 736,264 16605 mRNA_8238: 16606 - KOG2551 Phospholipase/carboxyhydrolase, at 736,874 to 737,919 16606 mRNA_8239: 16607 - K00101 E1.1.2.3, lldD L-lactate dehydrogenase (cytochrome), at 738,207 to 740,675 16607
Group Condition mRNA_8235 mRNA_8236 mRNA_8237 mRNA_8238 mRNA_8239
16603 16604 16605 16606 16607
growth on ypd YPD -0.7 +0.4 -0.1 -0.5 -0.6
facs on lipid accumulation Sorted No BODIPY Gate -0.3 -0.3 +0.1 -0.0 -0.5
facs on lipid accumulation Sorted High BODIPY Gate -0.3 -0.1 +0.7 -0.3 -0.4
ypd buoyancy 40 hrs High Buoyancy -0.3 +0.2 -0.5 -0.1 +0.3
ypd buoyancy 40 hrs Median Buoyancy -0.2 +0.3 -0.3 -0.3 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.3 +0.4 +0.5 -0.2 -0.4
ypd buoyancy 40 hrs No Buoyancy Separation +0.6 -0.4 -0.5 +0.3 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 +0.2 +0.2 +0.0 +0.0
growth on oleic acid YNB Glucose +0.2 -0.2 +0.4 -0.3 +0.0
auxotrophy YNB Glucose 75mM Methionine -0.2 +0.2 +0.4 +0.2 -0.3
auxotrophy YNB Glucose +0.0 +0.2 +0.5 -0.1 -0.3
growth on oleic acid YNB Oleic Acid +0.1 -0.0 +0.4 -0.2 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.4 -0.1 -0.1 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 +0.1 +0.1 +0.2 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.6 +0.5 -0.4 +0.3 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 +0.4 +0.3 +0.2 -0.2
auxotrophy Dropout Complete +0.0 +0.6 +0.6 -0.3 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 -0.1 +1.2 -0.2 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.3 +0.7 +0.0 +0.0
growth on ricinoleic acid YNB Glucose +0.3 +0.0 +1.0 +0.1 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -0.2 +1.3 -0.1 +0.2
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