Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16605 and 16606 are separated by 609 nucleotides16606 and 16607 are separated by 287 nucleotides16607 and 16608 are separated by 300 nucleotides16608 and 16609 are separated by 179 nucleotides mRNA_8237: 16605 - K04345 PKA protein kinase A, at 734,227 to 736,264 16605 mRNA_8238: 16606 - KOG2551 Phospholipase/carboxyhydrolase, at 736,874 to 737,919 16606 mRNA_8239: 16607 - K00101 E1.1.2.3, lldD L-lactate dehydrogenase (cytochrome), at 738,207 to 740,675 16607 mRNA_8240: 16608 - K14350 AADACL2 arylacetamide deacetylase-like 2, at 740,976 to 744,696 16608 mRNA_8241: 16609 - Hypothetical Protein, at 744,876 to 746,640 16609
Group Condition mRNA_8237 mRNA_8238 mRNA_8239 mRNA_8240 mRNA_8241
16605 16606 16607 16608 16609
growth on ypd YPD -0.1 -0.5 -0.6 +0.1 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 +0.3 +0.1 -0.4 -0.4
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.0 -0.5 +0.0 -0.0
ypd buoyancy 40 hrs Median Buoyancy -0.3 -0.3 +0.1 -0.1 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.5 -0.1 +0.3 -0.1 +0.2
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.1 +0.2 -0.2 +0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.7 -0.3 -0.4 -0.1 +0.1
growth on oleic acid YNB Glucose +0.4 -0.3 +0.0 +0.4 -0.4
growth on oleic acid YNB Oleic Acid +0.4 -0.2 +0.1 +0.4 -0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 +0.3 -0.2 +0.2 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.5 -0.2 -0.4 +0.2 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 +0.0 +0.0 +0.2 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.2 -0.2 +0.3 +0.1
auxotrophy YNB Glucose +0.5 -0.1 -0.3 +0.2 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 +0.2 -0.2 +0.2 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.7 +0.0 +0.0 +0.2 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +1.2 -0.2 -0.0 +0.4 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.4 +0.2 -0.3 +0.5 +0.5
auxotrophy Dropout Complete +0.6 -0.3 -0.0 +0.2 +0.8
growth on ricinoleic acid YNB Glucose +1.0 +0.1 +0.0 +0.3 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +1.3 -0.1 +0.2 +0.5 -0.1
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