Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16606 and 16607 are separated by 287 nucleotides16607 and 16608 are separated by 300 nucleotides16608 and 16609 are separated by 179 nucleotides16609 and 16610 are separated by 299 nucleotides mRNA_8238: 16606 - KOG2551 Phospholipase/carboxyhydrolase, at 736,874 to 737,919 16606 mRNA_8239: 16607 - K00101 E1.1.2.3, lldD L-lactate dehydrogenase (cytochrome), at 738,207 to 740,675 16607 mRNA_8240: 16608 - K14350 AADACL2 arylacetamide deacetylase-like 2, at 740,976 to 744,696 16608 mRNA_8241: 16609 - Hypothetical Protein, at 744,876 to 746,640 16609 mRNA_8242: 16610 - KOG3319 Predicted membrane protein, at 746,940 to 748,080 16610
Group Condition mRNA_8238 mRNA_8239 mRNA_8240 mRNA_8241 mRNA_8242
16606 16607 16608 16609 16610
facs on lipid accumulation Sorted High BODIPY Gate -0.3 -0.4 -0.1 +0.1 -1.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 +0.0 +0.2 -0.0 -1.9
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 +0.1 -0.4 -0.4 -0.9
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 -0.0 +0.4 -0.2 -0.6
growth on ypd YPD -0.5 -0.6 +0.1 -0.3 +0.7
growth on oleic acid YNB Glucose -0.3 +0.0 +0.4 -0.4 -0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.0 -0.5 +0.0 -0.0 +0.3
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.4 +0.2 +0.3 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 +0.2 +0.5 -0.1 -0.5
growth on oleic acid YNB Oleic Acid -0.2 +0.1 +0.4 -0.6 +0.4
ypd buoyancy 40 hrs Median Buoyancy -0.3 +0.1 -0.1 +0.1 +0.5
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.2 -0.2 +0.0 +0.7
growth on ricinoleic acid YNB Glucose +0.1 +0.0 +0.3 +0.2 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +0.0 +0.2 -0.3 +1.0
auxotrophy YNB Glucose 75mM Methionine +0.2 -0.3 +0.5 +0.5 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.3 -0.1 +0.2 +0.8
auxotrophy YNB Glucose -0.1 -0.3 +0.2 +0.3 +1.2
auxotrophy Dropout Complete -0.3 -0.0 +0.2 +0.8 +0.7
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 -0.2 +0.2 +0.2 +1.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -0.2 +0.3 +0.1 +1.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 -0.2 +0.2 +0.2 +1.4
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