Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16618 and 16619 are separated by 652 nucleotides16619 and 16620 are separated by 569 nucleotides16620 and 16621 are separated by 705 nucleotides16621 and 16622 are separated by 383 nucleotides mRNA_8250: 16618 - K17069 MET17 O-acetylhomoserine/O-acetylserine sulfhydrylase, at 764,459 to 766,227 16618 mRNA_8251: 16619 - HMMPfam-SacI homology domain-PF02383,ProSiteProfiles-Sac phosphatase domain profile.-PS50275, at 766,880 to 770,400 16619 mRNA_8252: 16620 - HMMPfam-Domain of unknown function (DUF4451)-PF14616, at 770,970 to 773,846 16620 mRNA_8253: 16621 - BLAST putative protein [Melanopsichium pennsylvanicum 4], at 774,552 to 775,844 16621 mRNA_8254: 16622 - HMMPfam-Stc1 domain-PF12898, at 776,228 to 777,333 16622
Group Condition mRNA_8250 mRNA_8251 mRNA_8252 mRNA_8253 mRNA_8254
16618 16619 16620 16621 16622
facs on lipid accumulation Sorted High BODIPY Gate +0.4 -0.6 N.D. -0.7 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 +0.4 N.D. -1.2 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 -0.4 N.D. +0.3 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.3 -0.1 N.D. -0.6 +0.1
auxotrophy YNB Glucose +0.2 +0.2 N.D. -0.7 -0.1
ypd buoyancy 40 hrs High Buoyancy -0.3 +0.3 N.D. -0.2 +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.2 N.D. -0.2 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 +0.3 N.D. -0.4 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 +0.3 N.D. -0.1 -0.0
auxotrophy YNB Glucose 75mM Methionine +0.2 +0.1 N.D. -0.3 +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.3 N.D. -0.1 +0.0
growth on ricinoleic acid YNB Glucose +0.4 -0.1 N.D. -0.6 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 +0.1 N.D. +0.4 -0.1
auxotrophy Dropout Complete +0.2 +0.2 N.D. -0.2 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 +0.3 N.D. +0.5 -0.1
growth on oleic acid YNB Glucose +0.2 +0.1 N.D. +0.4 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 +0.0 N.D. +0.3 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 +0.2 N.D. +0.3 -0.1
growth on ypd YPD +0.1 +0.3 N.D. +0.4 +0.2
growth on oleic acid YNB Oleic Acid +0.2 +0.2 N.D. +0.5 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.4 +0.2 N.D. +0.2 +0.2
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