Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16626 and 16627 are separated by 163 nucleotides16627 and 16628 are separated by 361 nucleotides16628 and 16629 are separated by 399 nucleotides16629 and 16630 are separated by 189 nucleotides mRNA_8258: 16626 - Hypothetical Protein, at 783,598 to 784,234 16626 mRNA_8259: 16627 - HMMPfam-DENN (AEX-3) domain-PF02141,ProSiteProfiles-DENN domain profile.-PS50211,SMART-Domain found in a variety of signalling proteins, always encircled by uDENN and dDENN-SM00799, at 784,398 to 789,104 16627 mRNA_8260: 16628 - KOG2354 RNA Polymerase C (III) 37 kDa subunit, at 789,466 to 790,634 16628 mRNA_8261: 16629 - KOG0714 Molecular chaperone (DnaJ superfamily), at 791,034 to 792,171 16629 mRNA_8262: 16630 - K01958 PC, pyc pyruvate carboxylase, at 792,361 to 796,882 16630
Group Condition mRNA_8258 mRNA_8259 mRNA_8260 mRNA_8261 mRNA_8262
16626 16627 16628 16629 16630
auxotrophy YNB Glucose -0.4 -0.1 N.D. -0.5 -3.2
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.2 N.D. -0.6 -3.2
auxotrophy YNB Glucose 75mM Arginine -0.0 -0.1 N.D. -0.4 -3.5
growth on oleic acid YNB Glucose -0.6 +0.1 N.D. -0.2 -3.2
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.0 N.D. -0.3 -2.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy +1.0 -0.2 N.D. +0.3 -3.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.4 -0.4 N.D. +0.0 -3.6
growth on ricinoleic acid YNB Glucose -0.0 -0.0 N.D. +0.1 -2.4
facs on lipid accumulation Sorted No BODIPY Gate +0.6 +0.1 N.D. -0.0 -2.9
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +1.1 -0.2 N.D. -0.2 -3.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 -0.1 N.D. +0.2 -1.9
facs on lipid accumulation Sorted Low BODIPY Gate +0.7 +0.2 N.D. -0.2 -2.0
auxotrophy Dropout Complete -0.3 +0.0 N.D. -0.4 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +0.1 N.D. -0.5 +0.2
growth on ypd YPD +0.2 +0.2 N.D. -0.1 -0.4
ypd buoyancy 40 hrs No Buoyancy Separation -0.9 +0.0 N.D. +0.3 +0.6
growth on oleic acid YNB Oleic Acid -0.0 +0.1 N.D. +0.1 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 +0.1 N.D. -0.0 +0.4
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.3 N.D. +0.1 +0.4
ypd buoyancy 40 hrs Low Buoyancy +0.3 +0.3 N.D. +0.2 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.5 +0.2 N.D. +0.2 +0.2
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