Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16628 and 16629 are separated by 399 nucleotides16629 and 16630 are separated by 189 nucleotides16630 and 16631 are separated by 765 nucleotides16631 and 16632 are separated by 298 nucleotides mRNA_8260: 16628 - KOG2354 RNA Polymerase C (III) 37 kDa subunit, at 789,466 to 790,634 16628 mRNA_8261: 16629 - KOG0714 Molecular chaperone (DnaJ superfamily), at 791,034 to 792,171 16629 mRNA_8262: 16630 - K01958 PC, pyc pyruvate carboxylase, at 792,361 to 796,882 16630 mRNA_8263: 16631 - KOG1432 Predicted DNA repair exonuclease SIA1, at 797,648 to 800,551 16631 mRNA_8264: 16632 - KOG4754 Predicted phosphoglycerate mutase, at 800,850 to 802,295 16632
Group Condition mRNA_8260 mRNA_8261 mRNA_8262 mRNA_8263 mRNA_8264
16628 16629 16630 16631 16632
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.6 -3.2 +0.1 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.3 -3.8 -0.2 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.0 -3.6 -0.5 +0.2
auxotrophy YNB Glucose N.D. -0.5 -3.2 +0.2 -0.0
growth on oleic acid YNB Glucose N.D. -0.2 -3.2 -0.1 +0.0
auxotrophy YNB Glucose 75mM Arginine N.D. -0.4 -3.5 +0.2 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.2 -3.0 -0.1 +0.3
growth on ricinoleic acid YNB Glucose N.D. +0.1 -2.4 +0.1 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. -0.3 -2.5 +0.2 +0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.0 -2.9 +0.6 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.2 -2.0 -0.2 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.2 -1.9 -0.2 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.5 +0.2 -0.2 -0.2
growth on ypd YPD N.D. -0.1 -0.4 +0.5 -0.5
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.2 +0.2 -0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.2 +0.2 -0.2 +0.0
growth on oleic acid YNB Oleic Acid N.D. +0.1 -0.1 +0.3 +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 +0.4 -0.2 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.0 +0.4 +0.0 +0.2
auxotrophy Dropout Complete N.D. -0.4 -0.1 +0.6 +0.8
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.3 +0.6 +0.3 -0.3
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