Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16629 and 16630 are separated by 189 nucleotides16630 and 16631 are separated by 765 nucleotides16631 and 16632 are separated by 298 nucleotides16632 and 16633 are separated by 264 nucleotides mRNA_8261: 16629 - KOG0714 Molecular chaperone (DnaJ superfamily), at 791,034 to 792,171 16629 mRNA_8262: 16630 - K01958 PC, pyc pyruvate carboxylase, at 792,361 to 796,882 16630 mRNA_8263: 16631 - KOG1432 Predicted DNA repair exonuclease SIA1, at 797,648 to 800,551 16631 mRNA_8264: 16632 - KOG4754 Predicted phosphoglycerate mutase, at 800,850 to 802,295 16632 mRNA_8265: 16633 - KOG0444 Cytoskeletal regulator Flightless-I (contains leucine-rich and gelsolin repeats), at 802,560 to 804,214 16633
Group Condition mRNA_8261 mRNA_8262 mRNA_8263 mRNA_8264 mRNA_8265
16629 16630 16631 16632 16633
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 -3.8 -0.2 -0.2 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 -3.6 -0.5 +0.2 -0.1
auxotrophy YNB Glucose -0.5 -3.2 +0.2 -0.0 -0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.6 -3.2 +0.1 -0.3 +0.3
auxotrophy YNB Glucose 75mM Arginine -0.4 -3.5 +0.2 +0.2 -0.1
growth on oleic acid YNB Glucose -0.2 -3.2 -0.1 +0.0 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -3.0 -0.1 +0.3 -0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.0 -2.9 +0.6 +0.1 -0.1
growth on ricinoleic acid YNB Glucose +0.1 -2.4 +0.1 -0.2 +0.4
auxotrophy YNB Glucose 75mM Methionine -0.3 -2.5 +0.2 +0.3 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -2.0 -0.2 +0.4 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -1.9 -0.2 +0.1 -0.0
growth on ypd YPD -0.1 -0.4 +0.5 -0.5 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.5 +0.2 -0.2 -0.2 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.2 +0.2 -0.1 -0.2 -0.1
growth on oleic acid YNB Oleic Acid +0.1 -0.1 +0.3 +0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.2 -0.2 +0.0 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.4 -0.2 +0.2 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 +0.4 +0.0 +0.2 +0.1
auxotrophy Dropout Complete -0.4 -0.1 +0.6 +0.8 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 +0.6 +0.3 -0.3 +0.2
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