Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16639 and 16640 are separated by 338 nucleotides16640 and 16641 are separated by 342 nucleotides16641 and 16642 are separated by 427 nucleotides16642 and 16643 are separated by 212 nucleotides mRNA_8271: 16639 - K14397 NUDT21, CPSF5, CFIM25 cleavage and polyadenylation specificity factor subunit 5, at 814,047 to 815,109 16639 mRNA_8272: 16640 - K07750 E1.14.13.72, SC4MOL, ERG25 methylsterol monooxygenase, at 815,448 to 817,149 16640 mRNA_8273: 16641 - K01866 YARS, tyrS tyrosyl-tRNA synthetase, at 817,492 to 819,082 16641 mRNA_8274: 16642 - KOG4080 Mitochondrial ribosomal protein L32, at 819,510 to 820,015 16642 mRNA_8275: 16643 - K00102 dld, LDHD D-lactate dehydrogenase (cytochrome), at 820,228 to 822,470 16643
Group Condition mRNA_8271 mRNA_8272 mRNA_8273 mRNA_8274 mRNA_8275
16639 16640 16641 16642 16643
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 N.D. N.D. N.D. -1.4
facs on lipid accumulation Sorted High BODIPY Gate -0.5 N.D. N.D. N.D. -1.1
auxotrophy YNB Glucose 75mM Arginine -0.3 N.D. N.D. N.D. -1.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 N.D. N.D. N.D. -1.3
facs on lipid accumulation Sorted No BODIPY Gate -0.0 N.D. N.D. N.D. -1.3
auxotrophy YNB Glucose 75mM Methionine -0.1 N.D. N.D. N.D. -1.1
auxotrophy YNB Glucose -0.3 N.D. N.D. N.D. -0.9
growth on oleic acid YNB Glucose -0.2 N.D. N.D. N.D. -0.8
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. N.D. N.D. -0.9
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. N.D. N.D. -0.7
growth on ricinoleic acid YNB Glucose +0.1 N.D. N.D. N.D. -0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. N.D. N.D. -0.6
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 N.D. N.D. N.D. -0.1
auxotrophy Dropout Complete -0.1 N.D. N.D. N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. N.D. N.D. -0.0
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 N.D. N.D. N.D. -0.1
growth on oleic acid YNB Oleic Acid -0.3 N.D. N.D. N.D. +0.3
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. N.D. N.D. +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 N.D. N.D. N.D. -0.1
growth on ypd YPD +0.1 N.D. N.D. N.D. +0.1
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