Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16640 and 16641 are separated by 342 nucleotides16641 and 16642 are separated by 427 nucleotides16642 and 16643 are separated by 212 nucleotides16643 and 16644 are separated by 511 nucleotides mRNA_8272: 16640 - K07750 E1.14.13.72, SC4MOL, ERG25 methylsterol monooxygenase, at 815,448 to 817,149 16640 mRNA_8273: 16641 - K01866 YARS, tyrS tyrosyl-tRNA synthetase, at 817,492 to 819,082 16641 mRNA_8274: 16642 - KOG4080 Mitochondrial ribosomal protein L32, at 819,510 to 820,015 16642 mRNA_8275: 16643 - K00102 dld, LDHD D-lactate dehydrogenase (cytochrome), at 820,228 to 822,470 16643 mRNA_8276: 16644 - K06630 YWHAE 14-3-3 protein epsilon, at 822,982 to 824,358 16644
Group Condition mRNA_8272 mRNA_8273 mRNA_8274 mRNA_8275 mRNA_8276
16640 16641 16642 16643 16644
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. -0.9 -1.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. -1.4 -0.8
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. -1.3 +0.0
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. -1.1 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. -0.1 -0.8
auxotrophy YNB Glucose N.D. N.D. N.D. -0.9 +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. -1.1 +0.5
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. -0.5 +0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. -1.1 +0.7
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. -0.6 +0.2
growth on oleic acid YNB Glucose N.D. N.D. N.D. -0.8 +0.4
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. -0.0 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. -1.3 +1.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. -0.1 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. +0.1 +0.1
growth on ypd YPD N.D. N.D. N.D. +0.1 +0.1
auxotrophy Dropout Complete N.D. N.D. N.D. -0.1 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. -0.7 +0.9
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. -0.1 +0.6
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. +0.0 +0.5
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. +0.3 +1.1
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