Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16646 and 16647 are separated by 818 nucleotides16647 and 16648 are separated by 296 nucleotides16648 and 16649 are separated by 567 nucleotides16649 and 16650 are separated by 126 nucleotides mRNA_8278: 16646 - K16330 K16330 pseudouridylate synthase / pseudouridine kinase, at 825,362 to 828,181 16646 mRNA_8279: 16647 - BLAST Type-1 angiotensin II receptor-associated protein [Ch..., at 829,000 to 829,923 16647 mRNA_8280: 16648 - KOG2157 Predicted tubulin-tyrosine ligase, at 830,220 to 831,668 16648 mRNA_8281: 16649 - Hypothetical Protein, at 832,236 to 832,909 16649 mRNA_8282: 16650 - K11359 SPT7 transcriptional activator SPT7, at 833,036 to 836,352 16650
Group Condition mRNA_8278 mRNA_8279 mRNA_8280 mRNA_8281 mRNA_8282
16646 16647 16648 16649 16650
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -1.1 N.D. +0.8 -1.7
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 -0.9 N.D. -0.4 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 -0.4 N.D. -0.3 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 -0.2 N.D. -0.7 -0.5
growth on ypd YPD +0.1 -0.8 N.D. +0.3 -0.6
growth on oleic acid YNB Glucose -0.2 -0.2 N.D. -0.3 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 -0.5 N.D. +1.3 -1.2
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.2 N.D. -0.3 -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.5 N.D. +0.7 -0.8
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.3 N.D. +0.5 -0.8
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 +0.2 N.D. -0.9 +0.7
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.0 N.D. +0.1 -0.4
growth on ricinoleic acid YNB Glucose -0.1 -0.4 N.D. -0.3 +0.5
auxotrophy Dropout Complete +0.1 -0.5 N.D. -0.2 +0.3
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.6 N.D. -0.0 -0.7
auxotrophy YNB Glucose 75mM Arginine +0.0 -0.2 N.D. -0.2 +0.4
auxotrophy YNB Glucose -0.1 -0.3 N.D. -0.1 +1.0
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -0.3 N.D. +0.3 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.5 N.D. +0.3 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 +0.5 N.D. +0.5 -0.2
growth on oleic acid YNB Oleic Acid -0.1 +0.2 N.D. -0.3 +1.2
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