Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16649 and 16650 are separated by 126 nucleotides16650 and 16651 are separated by 1215 nucleotides16651 and 16652 are separated by 408 nucleotides16652 and 16653 are separated by 493 nucleotides mRNA_8281: 16649 - Hypothetical Protein, at 832,236 to 832,909 16649 mRNA_8282: 16650 - K11359 SPT7 transcriptional activator SPT7, at 833,036 to 836,352 16650 mRNA_8283: 16651 - HMMPfam-Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region-PF00645,PRINTS-Histone H5 signature-PR00624,ProSiteProfiles-Poly(ADP-ribose) polymerase zinc finger domain profile.-PS50064,SUPERFAMILY--SSF57716, at 837,568 to 839,019 16651 mRNA_8284: 16652 - K07023 K07023 putative hydrolases of HD superfamily, at 839,428 to 840,649 16652 mRNA_8285: 16653 - HMMPfam-PH domain-PF00169,ProSiteProfiles-PH domain profile.-PS50003,SMART-Pleckstrin homology domain.-SM00233,SUPERFAMILY--SSF50729, at 841,143 to 845,451 16653
Group Condition mRNA_8281 mRNA_8282 mRNA_8283 mRNA_8284 mRNA_8285
16649 16650 16651 16652 16653
facs on lipid accumulation Sorted Low BODIPY Gate -0.7 -0.5 +0.1 -0.3 N.D.
growth on ypd YPD +0.3 -0.6 -0.4 -0.4 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.0 -0.7 -0.5 +0.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.3 +0.1 -0.6 -0.7 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.4 -0.4 -0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 -0.3 +0.3 -0.4 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.8 -1.7 +0.4 -0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.5 -0.8 +0.4 -0.8 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.3 -0.4 -0.3 +0.5 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.3 +0.1 -0.1 -0.6 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.7 -0.8 +0.2 -0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.9 +0.7 -0.2 +0.6 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.3 -1.2 +0.6 -0.4 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 -0.3 +0.1 +0.8 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.2 +0.4 -0.1 +0.5 N.D.
growth on oleic acid YNB Glucose -0.3 -0.0 +0.3 +0.8 N.D.
growth on ricinoleic acid YNB Glucose -0.3 +0.5 +0.2 +0.5 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 -0.2 +0.5 +0.0 N.D.
auxotrophy Dropout Complete -0.2 +0.3 +0.3 +0.4 N.D.
auxotrophy YNB Glucose -0.1 +1.0 -0.0 +0.4 N.D.
growth on oleic acid YNB Oleic Acid -0.3 +1.2 +0.3 +0.3 N.D.
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