Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16650 and 16651 are separated by 1215 nucleotides16651 and 16652 are separated by 408 nucleotides16652 and 16653 are separated by 493 nucleotides16653 and 16654 are separated by 469 nucleotides mRNA_8282: 16650 - K11359 SPT7 transcriptional activator SPT7, at 833,036 to 836,352 16650 mRNA_8283: 16651 - HMMPfam-Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region-PF00645,PRINTS-Histone H5 signature-PR00624,ProSiteProfiles-Poly(ADP-ribose) polymerase zinc finger domain profile.-PS50064,SUPERFAMILY--SSF57716, at 837,568 to 839,019 16651 mRNA_8284: 16652 - K07023 K07023 putative hydrolases of HD superfamily, at 839,428 to 840,649 16652 mRNA_8285: 16653 - HMMPfam-PH domain-PF00169,ProSiteProfiles-PH domain profile.-PS50003,SMART-Pleckstrin homology domain.-SM00233,SUPERFAMILY--SSF50729, at 841,143 to 845,451 16653 mRNA_8286: 16654 - HMMPfam-GATA zinc finger-PF00320,ProSiteProfiles-GATA-type zinc finger domain profile.-PS50114,SMART-zinc finger binding to DNA consensus sequence [AT]GATA[AG]-SM00401,SUPERFAMILY--SSF57716, at 845,921 to 849,990 16654
Group Condition mRNA_8282 mRNA_8283 mRNA_8284 mRNA_8285 mRNA_8286
16650 16651 16652 16653 16654
facs on lipid accumulation Sorted High BODIPY Gate -0.8 +0.4 -0.8 N.D. -0.9
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -1.7 +0.4 -0.1 N.D. -0.4
growth on ypd YPD -0.6 -0.4 -0.4 N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 +0.3 -0.4 N.D. -1.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.8 +0.2 -0.2 N.D. -0.5
ypd buoyancy 40 hrs Median Buoyancy -0.4 -0.4 -0.1 N.D. -0.4
ypd buoyancy 40 hrs High Buoyancy -0.7 -0.5 +0.3 N.D. -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -1.2 +0.6 -0.4 N.D. -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 +0.1 -0.3 N.D. -0.4
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.6 -0.7 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.1 -0.6 N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine -0.4 -0.3 +0.5 N.D. +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 +0.5 +0.0 N.D. +0.3
auxotrophy YNB Glucose 75mM Arginine +0.4 -0.1 +0.5 N.D. +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 +0.1 +0.8 N.D. +0.3
growth on oleic acid YNB Glucose -0.0 +0.3 +0.8 N.D. +0.1
auxotrophy Dropout Complete +0.3 +0.3 +0.4 N.D. +0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.7 -0.2 +0.6 N.D. +0.3
growth on ricinoleic acid YNB Glucose +0.5 +0.2 +0.5 N.D. +0.4
auxotrophy YNB Glucose +1.0 -0.0 +0.4 N.D. +0.3
growth on oleic acid YNB Oleic Acid +1.2 +0.3 +0.3 N.D. +0.6
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