Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16653 and 16654 are separated by 469 nucleotides16654 and 16655 are separated by 862 nucleotides16655 and 16656 are separated by 569 nucleotides16656 and 16657 are separated by 1761 nucleotides mRNA_8285: 16653 - HMMPfam-PH domain-PF00169,ProSiteProfiles-PH domain profile.-PS50003,SMART-Pleckstrin homology domain.-SM00233,SUPERFAMILY--SSF50729, at 841,143 to 845,451 16653 mRNA_8286: 16654 - HMMPfam-GATA zinc finger-PF00320,ProSiteProfiles-GATA-type zinc finger domain profile.-PS50114,SMART-zinc finger binding to DNA consensus sequence [AT]GATA[AG]-SM00401,SUPERFAMILY--SSF57716, at 845,921 to 849,990 16654 mRNA_8287: 16655 - K10245 ELO2 fatty acid elongase 2, at 850,853 to 852,932 16655 mRNA_8288: 16656 - KOG4135 Predicted phosphoglucosamine acetyltransferase, at 853,502 to 854,309 16656 mRNA_8289: 16657 - KOG1601 GATA-4/5/6 transcription factors, at 856,071 to 857,914 16657
Group Condition mRNA_8285 mRNA_8286 mRNA_8287 mRNA_8288 mRNA_8289
16653 16654 16655 16656 16657
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.9 -0.3 -0.3 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.4 -0.1 -0.6 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 -0.1 +0.1 -1.5
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 -0.1 -0.1 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -1.1 +0.1 -0.1 +0.5
growth on oleic acid YNB Glucose N.D. +0.1 -0.6 -0.0 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. -0.3 +0.2 -0.1 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.3 -0.6 +0.0 -0.0
growth on ypd YPD N.D. -0.1 +0.0 +0.2 -0.1
growth on oleic acid YNB Oleic Acid N.D. +0.6 -0.5 +0.5 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.5 -0.5 -0.1 +1.2
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.4 +0.4 -0.0 +0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 +0.3 +0.1 -0.2
auxotrophy YNB Glucose N.D. +0.3 -0.4 +0.2 +0.2
growth on ricinoleic acid YNB Glucose N.D. +0.4 -0.3 +0.2 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.4 -0.7 -0.1 +1.5
auxotrophy Dropout Complete N.D. +0.0 -0.2 +0.3 +0.2
auxotrophy YNB Glucose 75mM Methionine N.D. +0.3 -0.3 +0.4 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.3 -0.2 -0.0 +0.7
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 -0.1 +0.5 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 -0.8 +0.2 +1.6
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