Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16657 and 16658 are separated by 278 nucleotides16658 and 16659 are separated by 214 nucleotides16659 and 16660 are separated by 475 nucleotides16660 and 16661 are separated by 275 nucleotides mRNA_8289: 16657 - KOG1601 GATA-4/5/6 transcription factors, at 856,071 to 857,914 16657 mRNA_8290: 16658 - K02327 POLD1 DNA polymerase delta subunit 1, at 858,193 to 863,054 16658 mRNA_8291: 16659 - K00851 E2.7.1.12, gntK, idnK gluconokinase, at 863,269 to 864,250 16659 mRNA_8292: 16660 - HMMPfam-OHCU decarboxylase-PF09349,SUPERFAMILY--SSF158694, at 864,726 to 865,561 16660 mRNA_8293: 16661 - HMMPfam-Arrestin (or S-antigen), N-terminal domain-PF00339, at 865,837 to 868,084 16661
Group Condition mRNA_8289 mRNA_8290 mRNA_8291 mRNA_8292 mRNA_8293
16657 16658 16659 16660 16661
growth on ricinoleic acid YNB Ricinoleic Acid -1.5 N.D. -0.0 -0.1 +0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.3 N.D. -0.4 -0.3 -0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.3 N.D. -0.2 -0.4 +0.1
growth on oleic acid YNB Oleic Acid -0.5 N.D. -0.1 -0.2 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 N.D. -0.4 -0.1 -0.5
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. +0.2 -0.3 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 N.D. +0.0 -0.1 +0.2
growth on ypd YPD -0.1 N.D. -0.1 +0.3 -0.3
ypd buoyancy 40 hrs Low Buoyancy -0.2 N.D. -0.0 -0.1 +0.3
ypd buoyancy 40 hrs Median Buoyancy +0.3 N.D. -0.1 +0.1 +0.0
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. -0.4 +0.2 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 N.D. -0.2 -0.0 +0.8
growth on oleic acid YNB Glucose +0.2 N.D. +0.3 -0.0 +0.2
growth on ricinoleic acid YNB Glucose -0.0 N.D. -0.2 +0.2 +0.6
auxotrophy Dropout Complete +0.2 N.D. +0.2 +0.1 +0.4
auxotrophy YNB Glucose +0.2 N.D. -0.0 +0.3 +0.4
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. +0.3 +0.3 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.7 N.D. +0.7 +0.0 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +1.5 N.D. +0.2 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +1.2 N.D. +0.5 -0.1 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.6 N.D. +0.0 -0.0 +0.6
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