Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16664 and 16665 are separated by 1827 nucleotides16665 and 16666 are separated by 313 nucleotides16666 and 16667 are separated by 627 nucleotides16667 and 16668 are separated by 52 nucleotides mRNA_8296: 16664 - K19951 TBC1D8_9 TBC1 domain family member 8/9, at 871,259 to 875,299 16664 mRNA_8297: 16665 - Hypothetical Protein, at 877,127 to 877,627 16665 mRNA_8298: 16666 - K02858 ribB, RIB3 3,4-dihydroxy 2-butanone 4-phosphate synthase, at 877,941 to 878,924 16666 mRNA_8299: 16667 - Hypothetical Protein, at 879,552 to 881,168 16667 mRNA_8300: 16668 - HMMPfam-2OG-Fe(II) oxygenase superfamily-PF13640,ProSiteProfiles-Fe(2+) 2-oxoglutarate dioxygenase domain profile.-PS51471,SMART-Prolyl 4-hydroxylase alpha subunit homologues.-SM00702, at 881,221 to 882,082 16668
Group Condition mRNA_8296 mRNA_8297 mRNA_8298 mRNA_8299 mRNA_8300
16664 16665 16666 16667 16668
facs on lipid accumulation Sorted High BODIPY Gate -0.7 -0.0 N.D. -0.4 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -1.4 -0.0 N.D. +0.2 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate -1.0 +0.1 N.D. +0.1 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 -0.4 N.D. +0.4 -0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.6 +0.0 N.D. +0.1 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.1 N.D. -0.3 +0.0
growth on oleic acid YNB Oleic Acid -0.0 -0.2 N.D. -0.0 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.1 N.D. -0.0 +0.0
auxotrophy YNB Glucose -0.3 +0.0 N.D. +0.1 +0.0
ypd buoyancy 40 hrs High Buoyancy -0.2 -0.2 N.D. +0.1 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.6 +0.2 N.D. +0.1 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 +0.2 N.D. -0.1 +0.3
growth on ricinoleic acid YNB Glucose -0.5 +0.3 N.D. +0.2 -0.0
auxotrophy YNB Glucose 75mM Methionine -0.4 +0.2 N.D. +0.2 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.3 -0.2 N.D. +0.1 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 -0.0 N.D. +0.1 +0.2
growth on ypd YPD -0.3 +0.1 N.D. +0.3 +0.2
growth on oleic acid YNB Glucose +0.1 +0.2 N.D. +0.0 -0.0
auxotrophy Dropout Complete -0.2 +0.2 N.D. +0.4 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 +0.1 N.D. +0.3 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 +0.4 N.D. +0.2 +0.0
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