Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16665 and 16666 are separated by 313 nucleotides16666 and 16667 are separated by 627 nucleotides16667 and 16668 are separated by 52 nucleotides16668 and 16669 are separated by 144 nucleotides mRNA_8297: 16665 - Hypothetical Protein, at 877,127 to 877,627 16665 mRNA_8298: 16666 - K02858 ribB, RIB3 3,4-dihydroxy 2-butanone 4-phosphate synthase, at 877,941 to 878,924 16666 mRNA_8299: 16667 - Hypothetical Protein, at 879,552 to 881,168 16667 mRNA_8300: 16668 - HMMPfam-2OG-Fe(II) oxygenase superfamily-PF13640,ProSiteProfiles-Fe(2+) 2-oxoglutarate dioxygenase domain profile.-PS51471,SMART-Prolyl 4-hydroxylase alpha subunit homologues.-SM00702, at 881,221 to 882,082 16668 mRNA_8301: 16669 - K20361 USO1 intracellular protein transport protein USO1, at 882,227 to 887,090 16669
Group Condition mRNA_8297 mRNA_8298 mRNA_8299 mRNA_8300 mRNA_8301
16665 16666 16667 16668 16669
facs on lipid accumulation Sorted High BODIPY Gate -0.0 N.D. -0.4 -0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. -0.3 +0.0 N.D.
growth on oleic acid YNB Oleic Acid -0.2 N.D. -0.0 +0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. -0.0 +0.0 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 N.D. +0.4 -0.0 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. +0.1 +0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. +0.1 +0.1 N.D.
auxotrophy YNB Glucose +0.0 N.D. +0.1 +0.0 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. +0.1 -0.1 N.D.
growth on oleic acid YNB Glucose +0.2 N.D. +0.0 -0.0 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 N.D. +0.2 +0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.0 N.D. +0.1 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 N.D. +0.1 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. -0.1 +0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. +0.1 +0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. +0.2 +0.1 N.D.
growth on ricinoleic acid YNB Glucose +0.3 N.D. +0.2 -0.0 N.D.
auxotrophy Dropout Complete +0.2 N.D. +0.4 -0.0 N.D.
growth on ypd YPD +0.1 N.D. +0.3 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. +0.3 +0.3 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 N.D. +0.2 +0.0 N.D.
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