Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16668 and 16669 are separated by 144 nucleotides16669 and 16670 are separated by 204 nucleotides16670 and 16671 are separated by 1358 nucleotides16671 and 16672 are separated by 544 nucleotides mRNA_8300: 16668 - HMMPfam-2OG-Fe(II) oxygenase superfamily-PF13640,ProSiteProfiles-Fe(2+) 2-oxoglutarate dioxygenase domain profile.-PS51471,SMART-Prolyl 4-hydroxylase alpha subunit homologues.-SM00702, at 881,221 to 882,082 16668 mRNA_8301: 16669 - K20361 USO1 intracellular protein transport protein USO1, at 882,227 to 887,090 16669 mRNA_8302: 16670 - HMMPfam-Major Facilitator Superfamily-PF07690,SUPERFAMILY--SSF103473, at 887,295 to 889,058 16670 mRNA_8303: 16671 - KOG1398 Uncharacterized conserved protein, at 890,417 to 892,180 16671 mRNA_8304: 16672 - K01336 E3.4.21.48 cerevisin, at 892,725 to 894,788 16672
Group Condition mRNA_8300 mRNA_8301 mRNA_8302 mRNA_8303 mRNA_8304
16668 16669 16670 16671 16672
facs on lipid accumulation Sorted High BODIPY Gate -0.2 N.D. +0.5 -0.3 -2.2
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. -0.1 -0.1 -1.3
growth on oleic acid YNB Oleic Acid +0.1 N.D. -0.5 -0.5 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 N.D. -0.6 -0.2 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. -0.3 -0.3 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 N.D. -0.2 -0.2 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 N.D. +0.1 -0.2 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 N.D. -0.2 -0.1 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. -0.5 -0.0 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 N.D. -0.5 +0.2 -0.5
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. -0.3 -0.5 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. -0.4 -0.2 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. +0.1 -0.0 -0.4
growth on oleic acid YNB Glucose -0.0 N.D. +0.0 -0.1 +0.1
auxotrophy YNB Glucose +0.0 N.D. +0.1 -0.2 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 N.D. -0.2 +0.6 -0.2
auxotrophy Dropout Complete -0.0 N.D. +0.0 -0.2 +0.4
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. +0.0 +0.1 -0.0
growth on ypd YPD +0.2 N.D. +0.0 +0.0 +0.0
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. +0.2 +0.2 +0.0
growth on ricinoleic acid YNB Glucose -0.0 N.D. +0.3 +0.1 +0.2
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