Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16677 and 16678 are separated by 426 nucleotides16678 and 16679 are separated by 356 nucleotides16679 and 16680 are separated by 59 nucleotides16680 and 16681 are separated by 222 nucleotides mRNA_8309: 16677 - K06965 PELO, DOM34, pelA protein pelota, at 900,721 to 902,755 16677 mRNA_8310: 16678 - K18752 TNPO1, IPO2, KPNB2 transportin-1, at 903,182 to 906,312 16678 mRNA_8311: 16679 - K03016 RPB8, POLR2H DNA-directed RNA polymerases I, II, and III subunit RPABC3, at 906,669 to 907,618 16679 mRNA_8312: 16680 - K17868 DPH7, RRT2 diphthine methyl ester acylhydrolase, at 907,678 to 909,336 16680 mRNA_8313: 16681 - K11541 URA2 carbamoyl-phosphate synthase / aspartate carbamoyltransferase, at 909,559 to 916,766 16681
Group Condition mRNA_8309 mRNA_8310 mRNA_8311 mRNA_8312 mRNA_8313
16677 16678 16679 16680 16681
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. -1.6 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. -1.5 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. -1.2 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. -1.1 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. -1.1 N.D.
auxotrophy Dropout Complete N.D. N.D. N.D. -1.0 N.D.
auxotrophy YNB Glucose N.D. N.D. N.D. -0.9 N.D.
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. -0.6 N.D.
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. -0.4 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. -0.4 N.D.
growth on oleic acid YNB Glucose N.D. N.D. N.D. -0.4 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. -0.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. -0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. -0.2 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. -0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. +0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. +0.3 N.D.
growth on ypd YPD N.D. N.D. N.D. +0.3 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.4 N.D.
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