Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16678 and 16679 are separated by 356 nucleotides16679 and 16680 are separated by 59 nucleotides16680 and 16681 are separated by 222 nucleotides16681 and 16682 are separated by 933 nucleotides mRNA_8310: 16678 - K18752 TNPO1, IPO2, KPNB2 transportin-1, at 903,182 to 906,312 16678 mRNA_8311: 16679 - K03016 RPB8, POLR2H DNA-directed RNA polymerases I, II, and III subunit RPABC3, at 906,669 to 907,618 16679 mRNA_8312: 16680 - K17868 DPH7, RRT2 diphthine methyl ester acylhydrolase, at 907,678 to 909,336 16680 mRNA_8313: 16681 - K11541 URA2 carbamoyl-phosphate synthase / aspartate carbamoyltransferase, at 909,559 to 916,766 16681 mRNA_8314: 16682 - K03321 TC.SULP sulfate permease, SulP family, at 917,700 to 921,050 16682
Group Condition mRNA_8310 mRNA_8311 mRNA_8312 mRNA_8313 mRNA_8314
16678 16679 16680 16681 16682
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -1.6 N.D. +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -1.5 N.D. -0.0
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -1.2 N.D. -0.3
auxotrophy YNB Glucose N.D. N.D. -0.9 N.D. -0.4
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -1.1 N.D. -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -1.1 N.D. +0.1
auxotrophy Dropout Complete N.D. N.D. -1.0 N.D. +0.1
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.6 N.D. -0.1
growth on oleic acid YNB Glucose N.D. N.D. -0.4 N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.2 N.D. -0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.4 N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.4 N.D. +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.4 N.D. -0.7
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.1 N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.3 N.D. -0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.2 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.0 N.D. -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.3 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.3 N.D. +0.4
growth on ypd YPD N.D. N.D. +0.3 N.D. -0.0
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