Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16693 and 16694 are separated by 810 nucleotides16694 and 16695 are separated by 578 nucleotides16695 and 16696 are separated by 186 nucleotides16696 and 16697 are separated by 193 nucleotides mRNA_8325: 16693 - KOG1688 Golgi proteins involved in ER retention (RER), at 945,635 to 946,237 16693 mRNA_8326: 16694 - Hypothetical Protein, at 947,048 to 947,790 16694 mRNA_8327: 16695 - K10251 HSD17B12, KAR, IFA38 17beta-estradiol 17-dehydrogenase / very-long-chain 3-oxoacyl-CoA reductase, at 948,369 to 949,936 16695 mRNA_8328: 16696 - Hypothetical Protein, at 950,123 to 950,641 16696 mRNA_8329: 16697 - KOG0527 HMG-box transcription factor, at 950,835 to 951,817 16697
Group Condition mRNA_8325 mRNA_8326 mRNA_8327 mRNA_8328 mRNA_8329
16693 16694 16695 16696 16697
buoyancy on lipid accumulation 40 hrs High Buoyancy -3.1 +0.2 N.D. -0.4 -0.5
growth on ypd YPD -1.5 -0.4 N.D. -0.3 -0.7
facs on lipid accumulation Sorted Low BODIPY Gate -1.8 -0.1 N.D. -0.3 -0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -1.1 +0.4 N.D. -0.5 -1.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 -0.5 N.D. -1.7 +0.4
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.3 N.D. -0.5 -1.1
facs on lipid accumulation Sorted High BODIPY Gate -0.6 +0.0 N.D. -0.8 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.7 +0.5 N.D. -0.2 -0.9
ypd buoyancy 40 hrs Median Buoyancy -0.3 -0.1 N.D. -0.3 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 +0.5 N.D. -0.4 -0.8
growth on oleic acid YNB Glucose +0.4 -0.2 N.D. -0.2 -0.7
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 +0.1 N.D. -0.2 -0.2
growth on oleic acid YNB Oleic Acid +0.5 -0.1 N.D. -0.4 -0.4
growth on ricinoleic acid YNB Glucose +0.1 +0.3 N.D. -0.1 -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 +0.3 N.D. -0.0 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.4 -0.2 N.D. -0.1 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.7 -0.0 N.D. -0.3 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.3 +0.5 N.D. -0.8 +0.6
auxotrophy YNB Glucose +1.2 -0.4 N.D. -0.2 +0.2
auxotrophy Dropout Complete +0.7 -0.1 N.D. +0.1 +0.7
ypd buoyancy 40 hrs High Buoyancy +0.9 +0.1 N.D. +0.0 +0.4
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