Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16697 and 16698 are separated by 65 nucleotides16698 and 16699 are separated by 2215 nucleotides16699 and 16700 are separated by 107 nucleotides16700 and 16701 are separated by 364 nucleotides mRNA_8329: 16697 - KOG0527 HMG-box transcription factor, at 950,835 to 951,817 16697 mRNA_8330: 16698 - BLAST clp1-like protein [Schizophyllum commune H4-8], at 951,883 to 952,795 16698 mRNA_8331: 16699 - K02604 ORC2 origin recognition complex subunit 2, at 955,011 to 956,688 16699 mRNA_8332: 16700 - K10082 LMAN2, VIP36 lectin, mannose-binding 2, at 956,796 to 958,192 16700 mRNA_8333: 16701 - SUPERFAMILY--SSF55781, at 958,557 to 960,459 16701
Group Condition mRNA_8329 mRNA_8330 mRNA_8331 mRNA_8332 mRNA_8333
16697 16698 16699 16700 16701
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -1.2 +0.3 N.D. -0.2 -0.6
growth on ypd YPD -0.7 -0.0 N.D. -0.0 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.9 +0.4 N.D. -0.4 -0.0
auxotrophy YNB Glucose 75mM Arginine -1.1 -0.4 N.D. +0.6 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.5 +0.2 N.D. +0.2 -0.6
growth on oleic acid YNB Oleic Acid -0.4 -0.1 N.D. +0.3 -0.4
growth on oleic acid YNB Glucose -0.7 +0.1 N.D. +0.5 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.3 -0.1 N.D. +0.3 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 +0.2 N.D. +0.4 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 -0.7 N.D. +0.0 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.8 +0.4 N.D. +0.5 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.3 N.D. +0.7 +0.1
auxotrophy YNB Glucose +0.2 -0.5 N.D. +1.1 -0.2
growth on ricinoleic acid YNB Glucose -0.5 -0.2 N.D. +1.0 +0.3
ypd buoyancy 40 hrs High Buoyancy +0.4 -0.4 N.D. +0.4 +0.2
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.3 N.D. +0.7 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 -0.4 N.D. +1.1 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.1 +0.1 N.D. +1.3 -0.4
facs on lipid accumulation Sorted No BODIPY Gate +0.6 -0.2 N.D. +0.9 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.2 N.D. +1.3 +0.2
auxotrophy Dropout Complete +0.7 -0.3 N.D. +1.0 +0.1
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