Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16704 and 16705 are separated by 420 nucleotides16705 and 16706 are separated by 532 nucleotides16706 and 16707 are separated by 1076 nucleotides16707 and 16708 are separated by 115 nucleotides mRNA_8336: 16704 - KOG0134 NADH-flavin oxidoreductase/12-oxophytodienoate reductase, at 966,219 to 967,737 16704 mRNA_8337: 16705 - K01267 DNPEP aspartyl aminopeptidase, at 968,158 to 970,145 16705 mRNA_8338: 16706 - Hypothetical Protein, at 970,678 to 971,361 16706 mRNA_8339: 16707 - K06180 rluD 23S rRNA pseudouridine1911/1915/1917 synthase, at 972,438 to 973,571 16707 mRNA_8340: 16708 - K03254 EIF3A translation initiation factor 3 subunit A, at 973,687 to 977,357 16708
Group Condition mRNA_8336 mRNA_8337 mRNA_8338 mRNA_8339 mRNA_8340
16704 16705 16706 16707 16708
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.5 -1.9 +0.3 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 -0.4 -1.5 +0.4 N.D.
auxotrophy YNB Glucose -0.0 +0.0 -0.7 -0.3 N.D.
growth on ricinoleic acid YNB Glucose +0.2 -0.2 -1.0 +0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.1 -0.7 -0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 +0.2 +0.3 -0.8 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.1 -0.1 -0.2 N.D.
growth on oleic acid YNB Glucose +0.2 -0.1 -0.3 +0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.0 -0.1 +0.2 -0.1 N.D.
growth on oleic acid YNB Oleic Acid +0.3 -0.1 -0.3 +0.1 N.D.
auxotrophy Dropout Complete +0.1 +0.0 -0.1 +0.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 +0.2 -0.1 -0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.2 +0.4 -0.2 N.D.
growth on ypd YPD +0.1 -0.2 +0.0 +0.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.3 -0.0 +0.2 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.1 +0.1 +0.6 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.1 +0.1 +0.3 +0.2 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.4 -0.1 +0.1 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.2 -0.0 +0.6 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 +0.3 +0.5 +0.6 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.0 +0.6 +0.7 N.D.
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