Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16707 and 16708 are separated by 115 nucleotides16708 and 16709 are separated by 200 nucleotides16709 and 16710 are separated by 275 nucleotides16710 and 16711 are separated by 842 nucleotides mRNA_8339: 16707 - K06180 rluD 23S rRNA pseudouridine1911/1915/1917 synthase, at 972,438 to 973,571 16707 mRNA_8340: 16708 - K03254 EIF3A translation initiation factor 3 subunit A, at 973,687 to 977,357 16708 mRNA_8341: 16709 - K14569 BMS1 ribosome biogenesis protein BMS1, at 977,558 to 981,587 16709 mRNA_8342: 16710 - Hypothetical Protein, at 981,863 to 982,243 16710 mRNA_8343: 16711 - Hypothetical Protein, at 983,086 to 983,598 16711
Group Condition mRNA_8339 mRNA_8340 mRNA_8341 mRNA_8342 mRNA_8343
16707 16708 16709 16710 16711
ypd buoyancy 40 hrs No Buoyancy Separation -0.8 N.D. N.D. N.D. -0.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 N.D. N.D. N.D. -0.7
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. N.D. N.D. -0.6
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 N.D. N.D. N.D. -1.0
growth on ricinoleic acid YNB Glucose +0.3 N.D. N.D. N.D. -0.7
auxotrophy YNB Glucose -0.3 N.D. N.D. N.D. -0.0
growth on oleic acid YNB Oleic Acid +0.1 N.D. N.D. N.D. -0.4
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. N.D. N.D. -0.2
auxotrophy YNB Glucose 75mM Arginine -0.3 N.D. N.D. N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine -0.2 N.D. N.D. N.D. +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. N.D. N.D. -0.4
growth on oleic acid YNB Glucose +0.1 N.D. N.D. N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.2 N.D. N.D. N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. N.D. N.D. +0.2
auxotrophy Dropout Complete +0.1 N.D. N.D. N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. N.D. N.D. +0.2
growth on ypd YPD +0.3 N.D. N.D. N.D. +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.6 N.D. N.D. N.D. +0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.6 N.D. N.D. N.D. +0.9
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.6 N.D. N.D. N.D. +0.8
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.7 N.D. N.D. N.D. +0.9
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