Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16722 and 16723 are separated by 527 nucleotides16723 and 16724 are separated by 259 nucleotides16724 and 16725 are separated by 355 nucleotides16725 and 16726 are separated by 168 nucleotides mRNA_8354: 16722 - K07734 paiB transcriptional regulator, at 1,015,400 to 1,016,161 16722 mRNA_8355: 16723 - KOG2111 Uncharacterized conserved protein, contains WD40 repeats, at 1,016,689 to 1,018,583 16723 mRNA_8356: 16724 - K13985 NAPEPLD N-acyl-phosphatidylethanolamine-hydrolysing phospholipase D, at 1,018,843 to 1,020,921 16724 mRNA_8357: 16725 - K01739 metB cystathionine gamma-synthase, at 1,021,277 to 1,023,018 16725 mRNA_8358: 16726 - HMMPfam-Bin/amphiphysin/Rvs domain for vesicular trafficking-PF10455,HMMPfam-Fungal specific transcription factor domain-PF11951,SMART--SM00721,SUPERFAMILY--SSF103657, at 1,023,187 to 1,029,066 16726
Group Condition mRNA_8354 mRNA_8355 mRNA_8356 mRNA_8357 mRNA_8358
16722 16723 16724 16725 16726
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -4.9 +0.1 -0.6 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.6 -1.9 -0.1 +0.0 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 +0.0 +0.1 -1.2 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 -0.5 -0.1 -0.3 +0.1
auxotrophy YNB Glucose -0.8 -0.2 -0.1 +0.4 -0.1
growth on oleic acid YNB Oleic Acid -0.9 +0.3 -0.0 -0.1 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.6 -0.0 +0.1 -0.0 +0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -1.1 +0.1 +0.6 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 +0.7 -0.0 -0.6 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 +0.9 +0.1 -0.8 -0.0
ypd buoyancy 40 hrs High Buoyancy -0.7 -0.0 +0.2 +0.4 -0.0
auxotrophy YNB Glucose 75mM Arginine -0.3 +0.1 +0.0 +0.4 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.6 -0.6 +0.1 +0.1 +0.1
auxotrophy YNB Glucose 75mM Methionine -0.4 -0.1 +0.0 +0.6 +0.0
growth on oleic acid YNB Glucose -0.2 +0.4 +0.1 -0.1 +0.0
auxotrophy Dropout Complete -0.3 +0.2 +0.1 +0.5 -0.0
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.0 -0.0 +0.5 -0.0
growth on ricinoleic acid YNB Glucose +0.1 +0.6 +0.0 -0.0 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 +0.4 +0.1 -0.1 +0.2
growth on ypd YPD -0.3 +0.3 +0.4 +0.4 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.5 -0.1 +0.0 +0.9 -0.1
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