Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16724 and 16725 are separated by 355 nucleotides16725 and 16726 are separated by 168 nucleotides16726 and 16727 are separated by 89 nucleotides16727 and 16728 are separated by 44 nucleotides mRNA_8356: 16724 - K13985 NAPEPLD N-acyl-phosphatidylethanolamine-hydrolysing phospholipase D, at 1,018,843 to 1,020,921 16724 mRNA_8357: 16725 - K01739 metB cystathionine gamma-synthase, at 1,021,277 to 1,023,018 16725 mRNA_8358: 16726 - HMMPfam-Bin/amphiphysin/Rvs domain for vesicular trafficking-PF10455,HMMPfam-Fungal specific transcription factor domain-PF11951,SMART--SM00721,SUPERFAMILY--SSF103657, at 1,023,187 to 1,029,066 16726 mRNA_8359: 16727 - Hypothetical Protein, at 1,029,156 to 1,031,168 16727 mRNA_8360: 16728 - K02739 PSMB7 20S proteasome subunit beta 2, at 1,031,213 to 1,032,623 16728
Group Condition mRNA_8356 mRNA_8357 mRNA_8358 mRNA_8359 mRNA_8360
16724 16725 16726 16727 16728
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -1.2 -0.4 -0.0 -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -0.6 +0.1 -0.1 -0.6
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 -0.6 +0.0 -0.2 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -0.8 -0.0 -0.1 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.3 +0.1 +0.0 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.0 -0.0 +0.0 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.1 +0.2 +0.1 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.0 +0.0 -0.1 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.1 +0.1 -0.1 +0.1
growth on oleic acid YNB Glucose +0.1 -0.1 +0.0 -0.2 +0.4
growth on ricinoleic acid YNB Glucose +0.0 -0.0 +0.1 -0.1 +0.3
ypd buoyancy 40 hrs High Buoyancy +0.2 +0.4 -0.0 +0.2 -0.4
auxotrophy YNB Glucose -0.1 +0.4 -0.1 +0.1 +0.1
auxotrophy YNB Glucose 75mM Arginine +0.0 +0.4 -0.1 -0.0 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.5 -0.0 +0.4 -0.3
growth on oleic acid YNB Oleic Acid -0.0 -0.1 +0.1 -0.0 +0.7
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.6 +0.0 +0.2 -0.3
auxotrophy YNB Glucose 75mM Methionine +0.0 +0.6 +0.0 +0.0 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.9 -0.1 +0.2 -0.2
growth on ypd YPD +0.4 +0.4 +0.1 -0.2 +0.3
auxotrophy Dropout Complete +0.1 +0.5 -0.0 +0.1 +0.4
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