Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16810 and 16811 are separated by 230 nucleotides16811 and 16812 are separated by 219 nucleotides16812 and 16813 are separated by 907 nucleotides16813 and 16814 are separated by 253 nucleotides mRNA_8442: 16810 - K19852 KIN1_2 serine/threonine protein kinase KIN1/2, at 1,239,780 to 1,244,729 16810 mRNA_8443: 16811 - Hypothetical Protein, at 1,244,960 to 1,245,436 16811 mRNA_8444: 16812 - HMMPfam-HPP family-PF04982, at 1,245,656 to 1,246,720 16812 mRNA_8445: 16813 - KOG0691 Molecular chaperone (DnaJ superfamily), at 1,247,628 to 1,249,851 16813 mRNA_8446: 16814 - K01115 PLD1_2 phospholipase D1/2, at 1,250,105 to 1,254,255 16814
Group Condition mRNA_8442 mRNA_8443 mRNA_8444 mRNA_8445 mRNA_8446
16810 16811 16812 16813 16814
facs on lipid accumulation Sorted High BODIPY Gate -0.7 +0.7 -0.8 -0.9 -0.4
auxotrophy YNB Glucose +0.2 +0.4 -1.1 -0.3 -0.2
auxotrophy Dropout Complete +0.1 +0.4 -1.2 -0.2 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.0 -0.8 -0.2 -0.0
growth on ricinoleic acid YNB Glucose +0.2 +0.0 -0.5 -0.2 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.0 +0.4 -1.0 -0.1 +0.1
growth on ypd YPD -0.2 +0.4 -0.1 -0.2 -0.2
growth on oleic acid YNB Glucose +0.0 +0.5 -0.2 -0.3 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 +0.5 -0.6 -0.1 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.2 -0.1 +0.1 -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.1 +0.5 +0.0 -0.0 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.5 -0.2 -0.2 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 +0.4 -0.4 -0.2 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.1 +0.3 -0.1 -0.0
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.3 +0.1 -0.0 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 -0.3 +0.5 -0.2 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 +0.5 -0.0 -0.1 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 +0.2 -0.0 -0.1 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.4 +0.1 -0.0 +0.0
growth on oleic acid YNB Oleic Acid +0.1 +0.4 +0.1 -0.0 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.4 -0.1 +0.0 +0.2
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