Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16814 and 16815 are separated by 550 nucleotides16815 and 16816 are separated by 1058 nucleotides16816 and 16817 are separated by 160 nucleotides16817 and 16818 are separated by 3315 nucleotides mRNA_8446: 16814 - K01115 PLD1_2 phospholipase D1/2, at 1,250,105 to 1,254,255 16814 mRNA_8447: 16815 - K15429 TRM5, TRMT5 tRNA (guanine37-N1)-methyltransferase, at 1,254,806 to 1,256,692 16815 mRNA_8448: 16816 - HMMPfam-Protein of unknown function (DUF962)-PF06127, at 1,257,751 to 1,258,571 16816 mRNA_8449: 16817 - KOG0198 MEKK and related serine/threonine protein kinases, at 1,258,732 to 1,261,080 16817 mRNA_8450: 16818 - K06670 SCC1, MCD1, RAD21 cohesin complex subunit SCC1, at 1,264,396 to 1,266,785 16818
Group Condition mRNA_8446 mRNA_8447 mRNA_8448 mRNA_8449 mRNA_8450
16814 16815 16816 16817 16818
auxotrophy YNB Glucose -0.2 N.D. -0.0 -0.4 N.D.
growth on oleic acid YNB Glucose -0.2 N.D. -0.1 -0.1 N.D.
growth on oleic acid YNB Oleic Acid +0.0 N.D. -0.2 -0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.4 N.D. -0.2 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. -0.0 -0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 N.D. +0.0 -0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.0 N.D. +0.2 -0.4 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. +0.1 -0.2 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. +0.0 -0.1 N.D.
auxotrophy Dropout Complete -0.1 N.D. +0.2 -0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 N.D. +0.0 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 N.D. +0.0 -0.0 N.D.
growth on ypd YPD -0.2 N.D. +0.3 -0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.0 N.D. +0.1 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. -0.1 -0.0 N.D.
growth on ricinoleic acid YNB Glucose -0.2 N.D. +0.2 +0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. +0.2 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. +0.1 +0.0 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. +0.2 +0.3 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. +0.1 +0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. -0.1 +0.3 N.D.
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