Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16815 and 16816 are separated by 1058 nucleotides16816 and 16817 are separated by 160 nucleotides16817 and 16818 are separated by 3315 nucleotides16818 and 16819 are separated by 139 nucleotides mRNA_8447: 16815 - K15429 TRM5, TRMT5 tRNA (guanine37-N1)-methyltransferase, at 1,254,806 to 1,256,692 16815 mRNA_8448: 16816 - HMMPfam-Protein of unknown function (DUF962)-PF06127, at 1,257,751 to 1,258,571 16816 mRNA_8449: 16817 - KOG0198 MEKK and related serine/threonine protein kinases, at 1,258,732 to 1,261,080 16817 mRNA_8450: 16818 - K06670 SCC1, MCD1, RAD21 cohesin complex subunit SCC1, at 1,264,396 to 1,266,785 16818 mRNA_8451: 16819 - Hypothetical Protein, at 1,266,925 to 1,267,155 16819
Group Condition mRNA_8447 mRNA_8448 mRNA_8449 mRNA_8450 mRNA_8451
16815 16816 16817 16818 16819
auxotrophy YNB Glucose N.D. -0.0 -0.4 N.D. N.D.
growth on oleic acid YNB Oleic Acid N.D. -0.2 -0.2 N.D. N.D.
growth on oleic acid YNB Glucose N.D. -0.1 -0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.0 -0.2 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 -0.4 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.0 -0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 -0.0 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 -0.2 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.0 -0.1 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 -0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.0 -0.0 N.D. N.D.
auxotrophy Dropout Complete N.D. +0.2 -0.1 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 -0.1 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.2 +0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 +0.0 N.D. N.D.
growth on ypd YPD N.D. +0.3 -0.1 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.1 +0.3 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. +0.2 +0.1 N.D. N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.2 +0.1 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.2 +0.3 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.1 +0.3 N.D. N.D.
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