Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16817 and 16818 are separated by 3315 nucleotides16818 and 16819 are separated by 139 nucleotides16819 and 16820 are separated by 2390 nucleotides16820 and 16821 are separated by 800 nucleotides mRNA_8449: 16817 - KOG0198 MEKK and related serine/threonine protein kinases, at 1,258,732 to 1,261,080 16817 mRNA_8450: 16818 - K06670 SCC1, MCD1, RAD21 cohesin complex subunit SCC1, at 1,264,396 to 1,266,785 16818 mRNA_8451: 16819 - Hypothetical Protein, at 1,266,925 to 1,267,155 16819 mRNA_8452: 16820 - Hypothetical Protein, at 1,269,546 to 1,270,646 16820 mRNA_8453: 16821 - Hypothetical Protein, at 1,271,447 to 1,272,536 16821
Group Condition mRNA_8449 mRNA_8450 mRNA_8451 mRNA_8452 mRNA_8453
16817 16818 16819 16820 16821
facs on lipid accumulation Sorted No BODIPY Gate +0.3 N.D. N.D. -0.5 -0.8
facs on lipid accumulation Sorted High BODIPY Gate +0.2 N.D. N.D. -0.4 -0.8
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. N.D. -0.2 -0.9
auxotrophy YNB Glucose -0.4 N.D. N.D. -0.4 +0.2
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. N.D. -0.2 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. N.D. -0.3 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.4 N.D. N.D. -0.3 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 N.D. N.D. -0.3 -0.3
growth on oleic acid YNB Oleic Acid -0.2 N.D. N.D. -0.2 +0.2
auxotrophy YNB Glucose 75mM Methionine -0.2 N.D. N.D. -0.4 +0.4
auxotrophy Dropout Complete -0.1 N.D. N.D. -0.3 +0.3
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. N.D. +0.1 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. N.D. -0.0 -0.0
growth on ricinoleic acid YNB Glucose +0.1 N.D. N.D. +0.1 -0.1
growth on ypd YPD -0.1 N.D. N.D. -0.2 +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 N.D. N.D. -0.2 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 N.D. N.D. +0.2 +0.2
growth on oleic acid YNB Glucose -0.1 N.D. N.D. -0.1 +0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 N.D. N.D. -0.5 +1.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 N.D. N.D. -0.1 +0.9
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 N.D. N.D. -0.3 +1.5
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