Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16833 and 16834 are separated by 1971 nucleotides16834 and 16835 are separated by 257 nucleotides16835 and 16836 are separated by 313 nucleotides16836 and 16837 are separated by 197 nucleotides mRNA_8465: 16833 - K00293 LYS9 saccharopine dehydrogenase (NADP+, L-glutamate forming), at 1,297,471 to 1,300,237 16833 mRNA_8466: 16834 - KOG2112 Lysophospholipase, at 1,302,209 to 1,303,351 16834 mRNA_8467: 16835 - Hypothetical Protein, at 1,303,609 to 1,304,454 16835 mRNA_8468: 16836 - K00131 gapN glyceraldehyde-3-phosphate dehydrogenase (NADP+), at 1,304,768 to 1,306,783 16836 mRNA_8469: 16837 - K14654 RIB7, arfC 2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase, at 1,306,981 to 1,308,021 16837
Group Condition mRNA_8465 mRNA_8466 mRNA_8467 mRNA_8468 mRNA_8469
16833 16834 16835 16836 16837
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.2 -2.7 -0.4 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.4 -0.4 +0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. -0.4 -0.2 -0.0 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 -0.1 -0.2 N.D.
growth on oleic acid YNB Oleic Acid N.D. +0.0 +0.2 -0.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 -0.2 +0.2 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.5 +0.7 -0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 +0.3 -0.2 N.D.
auxotrophy Dropout Complete N.D. -0.1 +0.6 -0.3 N.D.
growth on oleic acid YNB Glucose N.D. +0.0 +0.3 -0.2 N.D.
auxotrophy YNB Glucose N.D. +0.3 +0.1 -0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.3 +0.2 -0.1 N.D.
growth on ypd YPD N.D. +0.3 +0.1 +0.1 N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.4 -0.0 +0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 +0.6 -0.3 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.4 +0.9 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.4 +0.9 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.4 +0.9 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 +1.1 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.5 +1.1 -0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.2 +1.3 +0.3 N.D.
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