Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16835 and 16836 are separated by 313 nucleotides16836 and 16837 are separated by 197 nucleotides16837 and 16838 are separated by 169 nucleotides16838 and 16839 are separated by 51 nucleotides mRNA_8467: 16835 - Hypothetical Protein, at 1,303,609 to 1,304,454 16835 mRNA_8468: 16836 - K00131 gapN glyceraldehyde-3-phosphate dehydrogenase (NADP+), at 1,304,768 to 1,306,783 16836 mRNA_8469: 16837 - K14654 RIB7, arfC 2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase, at 1,306,981 to 1,308,021 16837 mRNA_8470: 16838 - Hypothetical Protein, at 1,308,191 to 1,308,727 16838 mRNA_8471: 16839 - Hypothetical Protein, at 1,308,779 to 1,309,608 16839
Group Condition mRNA_8467 mRNA_8468 mRNA_8469 mRNA_8470 mRNA_8471
16835 16836 16837 16838 16839
ypd buoyancy 40 hrs No Buoyancy Separation -2.7 -0.4 N.D. -0.1 -3.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.9 -0.1 N.D. -1.6 -0.8
facs on lipid accumulation Sorted Low BODIPY Gate +0.7 -0.1 N.D. -2.5 +0.3
growth on oleic acid YNB Glucose +0.3 -0.2 N.D. -1.0 -0.1
growth on oleic acid YNB Oleic Acid +0.2 -0.3 N.D. -0.7 -0.1
growth on ypd YPD +0.1 +0.1 N.D. -1.7 +0.7
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.2 N.D. -0.6 +0.0
ypd buoyancy 40 hrs High Buoyancy -0.2 -0.0 N.D. -0.1 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.2 N.D. -0.7 +0.5
ypd buoyancy 40 hrs Median Buoyancy -0.4 +0.1 N.D. -0.1 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 -0.1 N.D. -0.6 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.9 +0.1 N.D. -0.5 -0.6
growth on ricinoleic acid YNB Glucose -0.0 +0.1 N.D. -0.4 +0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.9 -0.1 N.D. -1.1 +0.6
auxotrophy YNB Glucose +0.1 -0.2 N.D. +0.3 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.3 -0.2 N.D. +0.1 +0.3
facs on lipid accumulation Sorted High BODIPY Gate +1.3 +0.3 N.D. -1.3 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.1 +0.1 N.D. +0.2 -0.8
auxotrophy Dropout Complete +0.6 -0.3 N.D. +0.0 +0.5
auxotrophy YNB Glucose 75mM Arginine +0.6 -0.3 N.D. +0.7 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.1 -0.2 N.D. +0.3 -0.4
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