Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16849 and 16850 are separated by 695 nucleotides16850 and 16851 are separated by 700 nucleotides16851 and 16852 are separated by 222 nucleotides16852 and 16853 are separated by 223 nucleotides mRNA_8481: 16849 - Hypothetical Protein, at 1,325,084 to 1,327,878 16849 mRNA_8482: 16850 - K00854 xylB, XYLB xylulokinase, at 1,328,574 to 1,331,754 16850 mRNA_8483: 16851 - BLAST GA28568 gene product from transcript GA28568-RA [Rhiz..., at 1,332,455 to 1,336,946 16851 mRNA_8484: 16852 - KOG0725 Reductases with broad range of substrate specificities, at 1,337,169 to 1,338,536 16852 mRNA_8485: 16853 - K12946 SPCS1 signal peptidase complex subunit 1, at 1,338,760 to 1,339,317 16853
Group Condition mRNA_8481 mRNA_8482 mRNA_8483 mRNA_8484 mRNA_8485
16849 16850 16851 16852 16853
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 -0.1 +0.3 -0.2 -0.2
auxotrophy YNB Glucose +0.0 -0.1 -0.0 -0.2 -0.1
growth on ricinoleic acid YNB Glucose -0.1 +0.0 -0.0 +0.1 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 +0.1 +0.1 +0.2 -0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.0 -0.0 -0.0 -0.2 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.0 -0.0 +0.0 -0.1 +0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.0 -0.3 -0.0 +0.3
growth on ypd YPD +0.0 -0.0 -0.1 +0.0 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.4 -0.1 +0.1 -0.3 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 +0.1 +0.0 +0.2 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 +0.1 -0.1 -0.0 +0.6
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.1 -0.0 +0.1 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.0 +0.0 +0.1 +0.1
auxotrophy Dropout Complete +0.1 +0.0 +0.1 +0.1 +0.2
growth on oleic acid YNB Glucose -0.1 -0.0 +0.1 +0.3 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.2 +0.0 +0.1 +0.2
growth on oleic acid YNB Oleic Acid -0.0 +0.0 +0.1 +0.3 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 +0.3 +0.2 +0.4 -0.5
auxotrophy YNB Glucose 75mM Methionine +0.0 +0.1 +0.1 +0.1 +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.3 +0.2 +0.4 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 +0.3 +0.2 +0.4 -0.5
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