Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 16851 and 16852 are separated by 222 nucleotides16852 and 16853 are separated by 223 nucleotides16853 and 16854 are separated by 158 nucleotides16854 and 16855 are separated by 1419 nucleotides mRNA_8483: 16851 - BLAST GA28568 gene product from transcript GA28568-RA [Rhiz..., at 1,332,455 to 1,336,946 16851 mRNA_8484: 16852 - KOG0725 Reductases with broad range of substrate specificities, at 1,337,169 to 1,338,536 16852 mRNA_8485: 16853 - K12946 SPCS1 signal peptidase complex subunit 1, at 1,338,760 to 1,339,317 16853 mRNA_8486: 16854 - KOG1162 Predicted small molecule transporter, at 1,339,476 to 1,341,474 16854 mRNA_8487: 16855 - Hypothetical Protein, at 1,342,894 to 1,343,446 16855
Group Condition mRNA_8483 mRNA_8484 mRNA_8485 mRNA_8486 mRNA_8487
16851 16852 16853 16854 16855
auxotrophy YNB Glucose -0.0 -0.2 -0.1 N.D. N.D.
growth on ricinoleic acid YNB Glucose -0.0 +0.1 -0.2 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 -0.2 -0.2 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.3 +0.1 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.0 -0.2 +0.2 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine +0.0 -0.1 +0.0 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.3 -0.0 +0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.4 -0.5 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.4 -0.5 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +0.2 -0.2 N.D. N.D.
growth on ypd YPD -0.1 +0.0 +0.2 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.0 +0.1 +0.1 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.1 +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 +0.1 +0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.4 -0.3 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 +0.2 +0.1 N.D. N.D.
auxotrophy Dropout Complete +0.1 +0.1 +0.2 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 -0.0 +0.6 N.D. N.D.
growth on oleic acid YNB Oleic Acid +0.1 +0.3 +0.2 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.1 +0.4 N.D. N.D.
growth on oleic acid YNB Glucose +0.1 +0.3 +0.3 N.D. N.D.
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