Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8374 and 8375 are separated by 952 nucleotides8375 and 8376 are separated by 222 nucleotides8376 and 8377 are separated by 1976 nucleotides8377 and 8378 are separated by 267 nucleotides mRNA_6: 8374 - SUPERFAMILY--SSF47676, at 13,861 to 19,221 8374 mRNA_7: 8375 - HMMPfam-LIM-domain binding protein-PF01803,PRINTS-Proline rich extensin signature-PR01217, at 20,174 to 22,955 8375 mRNA_8: 8376 - K01194 TREH, treA, treF alpha,alpha-trehalase, at 23,178 to 26,734 8376 mRNA_9: 8377 - Hypothetical Protein, at 28,711 to 29,599 8377 mRNA_10: 8378 - Hypothetical Protein, at 29,867 to 30,741 8378
Group Condition mRNA_6 mRNA_7 mRNA_8 mRNA_9 mRNA_10
8374 8375 8376 8377 8378
facs on lipid accumulation Sorted High BODIPY Gate +0.0 -0.8 +0.1 +0.3 -1.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -1.1 -0.2 -0.4 +0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.2 -0.0 -0.2 -0.6
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 +0.1 +0.0 -0.2 -0.7
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.4 +0.1 -0.0 -0.5
auxotrophy YNB Glucose 75mM Methionine -0.3 -0.4 +0.2 +0.0 -0.1
growth on ricinoleic acid YNB Glucose +0.1 -0.4 -0.0 -0.3 +0.2
auxotrophy YNB Glucose 75mM Arginine -0.4 -0.6 +0.1 +0.2 +0.3
growth on oleic acid YNB Oleic Acid -0.1 -0.4 +0.2 +0.3 -0.4
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 +0.1 -0.3 -0.2 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -0.4 -0.2 -0.4 +0.3
ypd buoyancy 40 hrs High Buoyancy +0.2 -0.0 +0.2 -0.0 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.3 -0.0 +0.1 -0.1
auxotrophy Dropout Complete -0.2 -0.5 +0.1 +0.2 +0.3
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.1 -0.1 +0.0 +0.0
growth on oleic acid YNB Glucose -0.0 -0.2 +0.2 +0.2 -0.1
auxotrophy YNB Glucose -0.3 -0.5 +0.2 +0.4 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.0 +0.1 +0.3 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 -0.6 +0.1 +0.1 +0.2
growth on ypd YPD -0.2 +0.3 +0.1 -0.0 +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 -0.0 -0.1 +0.3 +0.3
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