Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8381 and 8382 are separated by 1636 nucleotides8382 and 8383 are separated by 1069 nucleotides8383 and 8384 are separated by 242 nucleotides8384 and 8385 are separated by 2737 nucleotides mRNA_13: 8381 - BLAST putative transmembrane protein [Rhizoctonia solani 123E], at 33,714 to 35,045 8381 mRNA_14: 8382 - K07199 PRKAB 5'-AMP-activated protein kinase, regulatory beta subunit, at 36,682 to 38,243 8382 mRNA_15: 8383 - K02321 POLA2 DNA polymerase alpha subunit B, at 39,313 to 41,292 8383 mRNA_16: 8384 - K03407 cheA two-component system, chemotaxis family, sensor kinase CheA, at 41,535 to 45,882 8384 mRNA_17: 8385 - K00856 E2.7.1.20, ADK adenosine kinase, at 48,620 to 50,142 8385
Group Condition mRNA_13 mRNA_14 mRNA_15 mRNA_16 mRNA_17
8381 8382 8383 8384 8385
growth on ricinoleic acid YNB Ricinoleic Acid -1.8 -2.3 N.D. +0.1 N.D.
growth on oleic acid YNB Oleic Acid -0.6 -2.1 N.D. -0.0 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.9 -0.9 N.D. -0.0 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.3 -1.3 N.D. +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 -1.2 N.D. -0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -1.0 N.D. +0.1 N.D.
growth on oleic acid YNB Glucose +0.1 -1.0 N.D. -0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 -0.9 N.D. +0.2 N.D.
growth on ricinoleic acid YNB Glucose -0.5 -0.0 N.D. -0.1 N.D.
growth on ypd YPD -0.0 -0.5 N.D. -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.6 N.D. +0.0 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.7 N.D. +0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.5 N.D. +0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 -0.1 N.D. +0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.4 N.D. +0.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 -0.8 N.D. +0.4 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 -0.7 N.D. +0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.4 +0.5 N.D. +0.1 N.D.
auxotrophy Dropout Complete +0.0 +0.0 N.D. +0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.2 +0.3 N.D. +0.2 N.D.
auxotrophy YNB Glucose -0.3 +0.5 N.D. +0.3 N.D.
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