Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8382 and 8383 are separated by 1069 nucleotides8383 and 8384 are separated by 242 nucleotides8384 and 8385 are separated by 2737 nucleotides8385 and 8386 are separated by 330 nucleotides mRNA_14: 8382 - K07199 PRKAB 5'-AMP-activated protein kinase, regulatory beta subunit, at 36,682 to 38,243 8382 mRNA_15: 8383 - K02321 POLA2 DNA polymerase alpha subunit B, at 39,313 to 41,292 8383 mRNA_16: 8384 - K03407 cheA two-component system, chemotaxis family, sensor kinase CheA, at 41,535 to 45,882 8384 mRNA_17: 8385 - K00856 E2.7.1.20, ADK adenosine kinase, at 48,620 to 50,142 8385 mRNA_18: 8386 - KOG1515 Arylacetamide deacetylase, at 50,473 to 51,921 8386
Group Condition mRNA_14 mRNA_15 mRNA_16 mRNA_17 mRNA_18
8382 8383 8384 8385 8386
growth on ricinoleic acid YNB Ricinoleic Acid -2.3 N.D. +0.1 N.D. +0.1
growth on oleic acid YNB Oleic Acid -2.1 N.D. -0.0 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.2 N.D. -0.0 N.D. -0.0
facs on lipid accumulation Sorted High BODIPY Gate -1.3 N.D. +0.1 N.D. +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -1.0 N.D. +0.1 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.7 N.D. +0.1 N.D. -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.9 N.D. -0.0 N.D. -0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.8 N.D. +0.4 N.D. -0.4
growth on ypd YPD -0.5 N.D. -0.1 N.D. -0.2
growth on oleic acid YNB Glucose -1.0 N.D. -0.0 N.D. +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.9 N.D. +0.2 N.D. +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.6 N.D. +0.0 N.D. -0.0
ypd buoyancy 40 hrs High Buoyancy -0.5 N.D. +0.1 N.D. -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.7 N.D. +0.1 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.4 N.D. +0.1 N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. +0.1 N.D. -0.3
growth on ricinoleic acid YNB Glucose -0.0 N.D. -0.1 N.D. -0.1
auxotrophy Dropout Complete +0.0 N.D. +0.2 N.D. -0.2
auxotrophy YNB Glucose 75mM Arginine +0.5 N.D. +0.1 N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. +0.2 N.D. +0.1
auxotrophy YNB Glucose +0.5 N.D. +0.3 N.D. +0.1
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