Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8383 and 8384 are separated by 242 nucleotides8384 and 8385 are separated by 2737 nucleotides8385 and 8386 are separated by 330 nucleotides8386 and 8387 are separated by 379 nucleotides mRNA_15: 8383 - K02321 POLA2 DNA polymerase alpha subunit B, at 39,313 to 41,292 8383 mRNA_16: 8384 - K03407 cheA two-component system, chemotaxis family, sensor kinase CheA, at 41,535 to 45,882 8384 mRNA_17: 8385 - K00856 E2.7.1.20, ADK adenosine kinase, at 48,620 to 50,142 8385 mRNA_18: 8386 - KOG1515 Arylacetamide deacetylase, at 50,473 to 51,921 8386 mRNA_19: 8387 - KOG1399 Flavin-containing monooxygenase, at 52,301 to 54,654 8387
Group Condition mRNA_15 mRNA_16 mRNA_17 mRNA_18 mRNA_19
8383 8384 8385 8386 8387
growth on ypd YPD N.D. -0.1 N.D. -0.2 -0.3
auxotrophy Dropout Complete N.D. +0.2 N.D. -0.2 -0.4
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 N.D. -0.1 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.0 N.D. -0.0 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 N.D. -0.1 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.0 N.D. -0.0 -0.0
growth on ricinoleic acid YNB Glucose N.D. -0.1 N.D. -0.1 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.1 N.D. -0.3 +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.1 N.D. -0.3 +0.2
growth on oleic acid YNB Oleic Acid N.D. -0.0 N.D. +0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 N.D. -0.0 +0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 N.D. -0.1 +0.0
auxotrophy YNB Glucose N.D. +0.3 N.D. +0.1 -0.3
growth on oleic acid YNB Glucose N.D. -0.0 N.D. +0.2 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.4 N.D. -0.4 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 N.D. +0.1 -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 N.D. -0.0 +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 N.D. +0.1 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 N.D. +0.1 +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 N.D. +0.1 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.2 N.D. +0.0 +0.3
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