Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8394 and 8395 are separated by 449 nucleotides8395 and 8396 are separated by 452 nucleotides8396 and 8397 are separated by 166 nucleotides8397 and 8398 are separated by 247 nucleotides mRNA_26: 8394 - K04499 RUVBL1, RVB1, INO80H RuvB-like protein 1 (pontin 52), at 68,110 to 69,853 8394 mRNA_27: 8395 - K00088 IMPDH, guaB IMP dehydrogenase, at 70,303 to 72,461 8395 mRNA_28: 8396 - Hypothetical Protein, at 72,914 to 73,928 8396 mRNA_29: 8397 - HMMPfam-Swi5-PF07061, at 74,095 to 74,821 8397 mRNA_30: 8398 - KOG2798 Putative trehalase, at 75,069 to 76,780 8398
Group Condition mRNA_26 mRNA_27 mRNA_28 mRNA_29 mRNA_30
8394 8395 8396 8397 8398
auxotrophy Dropout Complete N.D. N.D. -0.1 -0.5 -0.5
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.3 -0.2 -0.5
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.1 -0.1 -0.6
auxotrophy YNB Glucose N.D. N.D. +0.0 -0.1 -0.6
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.1 +0.0 -0.6
growth on oleic acid YNB Glucose N.D. N.D. -0.1 -0.1 -0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.1 +0.1 -0.7
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.2 -0.3 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.1 -0.2 -0.4
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.1 -0.0 -0.5
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.1 -0.2 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.1 +0.1 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.2 +0.2 -0.5
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.3 +0.1 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.1 +0.0 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.2 +0.4 -0.2
growth on ypd YPD N.D. N.D. +0.1 +0.1 +0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.4 -0.1 -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.0 +0.1 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.1 +0.2 +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.1 +0.4 +0.2
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