Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8398 and 8399 are separated by 84 nucleotides8399 and 8400 are separated by 294 nucleotides8400 and 8401 are separated by 355 nucleotides8401 and 8402 are separated by 145 nucleotides mRNA_30: 8398 - KOG2798 Putative trehalase, at 75,069 to 76,780 8398 mRNA_31: 8399 - K01520 dut, DUT dUTP pyrophosphatase, at 76,865 to 77,832 8399 mRNA_32: 8400 - HMMPfam-Pre-rRNA-processing protein TSR2-PF10273, at 78,127 to 78,849 8400 mRNA_33: 8401 - HMMPfam-Myosin-binding striated muscle assembly central-PF11701,SUPERFAMILY--SSF48371, at 79,205 to 81,384 8401 mRNA_34: 8402 - BLAST proteophosphoglycan 5 [Moniliophthora roreri MCA ..., at 81,530 to 84,325 8402
Group Condition mRNA_30 mRNA_31 mRNA_32 mRNA_33 mRNA_34
8398 8399 8400 8401 8402
facs on lipid accumulation Sorted High BODIPY Gate +0.2 N.D. N.D. +0.7 -3.5
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. N.D. -0.2 -2.2
ypd buoyancy 40 hrs Low Buoyancy -0.4 N.D. N.D. +0.6 -2.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.6 N.D. N.D. +1.8 -3.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. N.D. -0.6 -1.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.5 N.D. N.D. +2.1 -3.0
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. N.D. -0.1 -1.5
auxotrophy YNB Glucose 75mM Arginine -0.5 N.D. N.D. +0.4 -1.1
auxotrophy YNB Glucose -0.6 N.D. N.D. +0.6 -1.1
auxotrophy Dropout Complete -0.5 N.D. N.D. +0.8 -1.3
auxotrophy YNB Glucose 75mM Methionine -0.6 N.D. N.D. +0.6 -0.8
growth on oleic acid YNB Glucose -0.5 N.D. N.D. +0.9 -1.2
growth on ricinoleic acid YNB Glucose -0.5 N.D. N.D. +1.0 -1.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 N.D. N.D. -0.2 -0.1
growth on oleic acid YNB Oleic Acid -0.1 N.D. N.D. +1.3 -1.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.7 N.D. N.D. +0.2 +0.7
growth on ypd YPD +0.1 N.D. N.D. +0.7 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. N.D. -0.1 +1.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.5 N.D. N.D. +0.6 +1.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 N.D. N.D. +0.9 +0.9
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. N.D. +0.0 +2.3
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