Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8399 and 8400 are separated by 294 nucleotides8400 and 8401 are separated by 355 nucleotides8401 and 8402 are separated by 145 nucleotides8402 and 8403 are separated by 657 nucleotides mRNA_31: 8399 - K01520 dut, DUT dUTP pyrophosphatase, at 76,865 to 77,832 8399 mRNA_32: 8400 - HMMPfam-Pre-rRNA-processing protein TSR2-PF10273, at 78,127 to 78,849 8400 mRNA_33: 8401 - HMMPfam-Myosin-binding striated muscle assembly central-PF11701,SUPERFAMILY--SSF48371, at 79,205 to 81,384 8401 mRNA_34: 8402 - BLAST proteophosphoglycan 5 [Moniliophthora roreri MCA ..., at 81,530 to 84,325 8402 mRNA_35: 8403 - KOG1611 Predicted short chain-type dehydrogenase, at 84,983 to 86,013 8403
Group Condition mRNA_31 mRNA_32 mRNA_33 mRNA_34 mRNA_35
8399 8400 8401 8402 8403
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.7 -3.5 -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.2 -2.2 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.6 -2.2 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +1.8 -3.1 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.6 -1.1 +0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.1 -1.5 +0.3
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.4 -1.1 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +2.1 -3.0 +0.1
auxotrophy Dropout Complete N.D. N.D. +0.8 -1.3 -0.0
growth on oleic acid YNB Glucose N.D. N.D. +0.9 -1.2 -0.0
auxotrophy YNB Glucose N.D. N.D. +0.6 -1.1 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.2 -0.1 -0.0
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.6 -0.8 +0.0
growth on ricinoleic acid YNB Glucose N.D. N.D. +1.0 -1.0 -0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. +1.3 -1.1 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.2 +0.7 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.1 +1.2 -0.2
growth on ypd YPD N.D. N.D. +0.7 +0.1 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.6 +1.1 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.9 +0.9 +0.0
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.0 +2.3 +0.1
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