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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
8418 and 8419 are separated by 231 nucleotides
8419 and 8420 are separated by 53 nucleotides
8420 and 8421 are separated by 210 nucleotides
8421 and 8422 are separated by 453 nucleotides
mRNA_50: 8418 - KOG1611 Predicted short chain-type dehydrogenase, at 118,975 to 119,840
8418
mRNA_51: 8419 - K10392 KIF1 kinesin family member 1, at 120,072 to 126,065
8419
mRNA_52: 8420 - Hypothetical Protein, at 126,119 to 126,700
8420
mRNA_53: 8421 - K03014 RPB6, POLR2F DNA-directed RNA polymerases I, II, and III subunit RPABC2, at 126,911 to 127,593
8421
mRNA_54: 8422 - KOG1505 Lysophosphatidic acid acyltransferase LPAAT and related acyltransferases, at 128,047 to 129,356
8422
Group
Condition
mRNA
_50
mRNA
_51
mRNA
_52
mRNA
_53
mRNA
_54
8418
8419
8420
8421
8422
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.1
-0.9
-0.7
N.D.
-0.1
auxotrophy
YNB Glucose 75mM Arginine
-0.1
-0.6
-0.3
N.D.
-0.3
auxotrophy
YNB Glucose
-0.2
-0.4
-0.3
N.D.
-0.3
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.6
-0.1
-0.4
N.D.
-0.1
auxotrophy
Dropout Complete
-0.2
-0.4
-0.5
N.D.
-0.1
growth on ricinoleic acid
YNB Glucose
-0.2
-0.7
-0.4
N.D.
+0.0
auxotrophy
YNB Glucose 75mM Methionine
-0.0
-0.5
-0.3
N.D.
-0.4
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.4
-0.6
-0.1
N.D.
+0.1
ypd buoyancy
40 hrs No Buoyancy Separation
+0.5
+0.4
-0.8
N.D.
-0.9
facs on lipid accumulation
Sorted No BODIPY Gate
-0.2
+0.2
-0.5
N.D.
+0.0
ypd buoyancy
40 hrs Median Buoyancy
+0.2
-0.0
-0.3
N.D.
-0.2
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.3
-0.1
+0.3
N.D.
-0.1
ypd buoyancy
40 hrs High Buoyancy
-0.0
-0.1
-0.3
N.D.
+0.4
ypd buoyancy
40 hrs Low Buoyancy
+0.0
+0.1
-0.3
N.D.
+0.1
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.5
+0.2
+0.1
N.D.
+0.3
growth on oleic acid
YNB Glucose
-0.1
-0.4
-0.1
N.D.
+0.7
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.1
+0.2
+0.0
N.D.
-0.2
facs on lipid accumulation
Sorted High BODIPY Gate
-0.0
+0.3
+0.0
N.D.
+0.0
growth on ypd
YPD
+0.2
+0.0
-0.2
N.D.
+0.3
growth on oleic acid
YNB Oleic Acid
+0.1
-0.3
-0.0
N.D.
+0.8
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.1
+0.2
+0.3
N.D.
-0.1
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