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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
8420 and 8421 are separated by 210 nucleotides
8421 and 8422 are separated by 453 nucleotides
8422 and 8423 are separated by 108 nucleotides
8423 and 8424 are separated by 135 nucleotides
mRNA_52: 8420 - Hypothetical Protein, at 126,119 to 126,700
8420
mRNA_53: 8421 - K03014 RPB6, POLR2F DNA-directed RNA polymerases I, II, and III subunit RPABC2, at 126,911 to 127,593
8421
mRNA_54: 8422 - KOG1505 Lysophosphatidic acid acyltransferase LPAAT and related acyltransferases, at 128,047 to 129,356
8422
mRNA_55: 8423 - HMMPfam-Endonuclease/Exonuclease/phosphatase family-PF03372,SUPERFAMILY--SSF56219, at 129,465 to 130,938
8423
mRNA_56: 8424 - K09499 CCT7 T-complex protein 1 subunit eta, at 131,074 to 133,280
8424
Group
Condition
mRNA
_52
mRNA
_53
mRNA
_54
mRNA
_55
mRNA
_56
8420
8421
8422
8423
8424
ypd buoyancy
40 hrs No Buoyancy Separation
-0.8
N.D.
-0.9
-0.5
N.D.
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.4
N.D.
-0.1
-0.2
N.D.
auxotrophy
Dropout Complete
-0.5
N.D.
-0.1
-0.0
N.D.
auxotrophy
YNB Glucose 75mM Methionine
-0.3
N.D.
-0.4
+0.1
N.D.
auxotrophy
YNB Glucose
-0.3
N.D.
-0.3
+0.1
N.D.
auxotrophy
YNB Glucose 75mM Arginine
-0.3
N.D.
-0.3
+0.1
N.D.
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.7
N.D.
-0.1
+0.3
N.D.
ypd buoyancy
40 hrs Median Buoyancy
-0.3
N.D.
-0.2
+0.2
N.D.
facs on lipid accumulation
Sorted No BODIPY Gate
-0.5
N.D.
+0.0
+0.5
N.D.
ypd buoyancy
40 hrs Low Buoyancy
-0.3
N.D.
+0.1
+0.2
N.D.
growth on ricinoleic acid
YNB Glucose
-0.4
N.D.
+0.0
+0.4
N.D.
facs on lipid accumulation
Sorted High BODIPY Gate
+0.0
N.D.
+0.0
+0.2
N.D.
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.0
N.D.
-0.2
+0.5
N.D.
growth on ypd
YPD
-0.2
N.D.
+0.3
+0.4
N.D.
growth on oleic acid
YNB Glucose
-0.1
N.D.
+0.7
-0.1
N.D.
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.3
N.D.
-0.1
+0.3
N.D.
ypd buoyancy
40 hrs High Buoyancy
-0.3
N.D.
+0.4
+0.5
N.D.
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.3
N.D.
-0.1
+0.4
N.D.
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.1
N.D.
+0.3
+0.3
N.D.
growth on oleic acid
YNB Oleic Acid
-0.0
N.D.
+0.8
-0.0
N.D.
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.1
N.D.
+0.1
+0.7
N.D.
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