Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8421 and 8422 are separated by 453 nucleotides8422 and 8423 are separated by 108 nucleotides8423 and 8424 are separated by 135 nucleotides8424 and 8425 are separated by 361 nucleotides mRNA_53: 8421 - K03014 RPB6, POLR2F DNA-directed RNA polymerases I, II, and III subunit RPABC2, at 126,911 to 127,593 8421 mRNA_54: 8422 - KOG1505 Lysophosphatidic acid acyltransferase LPAAT and related acyltransferases, at 128,047 to 129,356 8422 mRNA_55: 8423 - HMMPfam-Endonuclease/Exonuclease/phosphatase family-PF03372,SUPERFAMILY--SSF56219, at 129,465 to 130,938 8423 mRNA_56: 8424 - K09499 CCT7 T-complex protein 1 subunit eta, at 131,074 to 133,280 8424 mRNA_57: 8425 - K15176 CTR9 RNA polymerase-associated protein CTR9, at 133,642 to 138,012 8425
Group Condition mRNA_53 mRNA_54 mRNA_55 mRNA_56 mRNA_57
8421 8422 8423 8424 8425
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.9 -0.5 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. -0.4 +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 -0.2 N.D. N.D.
auxotrophy YNB Glucose N.D. -0.3 +0.1 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. -0.3 +0.1 N.D. N.D.
auxotrophy Dropout Complete N.D. -0.1 -0.0 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.2 +0.2 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.0 +0.2 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 +0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 +0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 +0.5 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 +0.4 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 +0.2 N.D. N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.0 +0.4 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.0 +0.5 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.3 +0.3 N.D. N.D.
growth on oleic acid YNB Glucose N.D. +0.7 -0.1 N.D. N.D.
growth on ypd YPD N.D. +0.3 +0.4 N.D. N.D.
growth on oleic acid YNB Oleic Acid N.D. +0.8 -0.0 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 +0.7 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. +0.4 +0.5 N.D. N.D.
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