Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8443 and 8444 are separated by 138 nucleotides8444 and 8445 are separated by 57 nucleotides8445 and 8446 are separated by 112 nucleotides8446 and 8447 are separated by 498 nucleotides mRNA_75: 8443 - K00606 panB 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 180,761 to 182,108 8443 mRNA_76: 8444 - K18164 NDUFAF7 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 7, at 182,247 to 184,330 8444 mRNA_77: 8445 - K13121 FRA10AC1 protein FRA10AC1, at 184,388 to 185,510 8445 mRNA_78: 8446 - HMMPfam-NADH-ubiquinone oxidoreductase B15 subunit (NDUFB4)-PF07225, at 185,623 to 186,207 8446 mRNA_79: 8447 - K04906 KCNH3 potassium voltage-gated channel Eag-related subfamily H member 3, at 186,706 to 188,932 8447
Group Condition mRNA_75 mRNA_76 mRNA_77 mRNA_78 mRNA_79
8443 8444 8445 8446 8447
growth on oleic acid YNB Glucose +0.1 N.D. N.D. N.D. -0.9
growth on ypd YPD +0.0 N.D. N.D. N.D. -0.7
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 N.D. N.D. N.D. +0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 N.D. N.D. N.D. -0.4
growth on ricinoleic acid YNB Glucose +0.0 N.D. N.D. N.D. -0.2
growth on oleic acid YNB Oleic Acid +0.3 N.D. N.D. N.D. -0.3
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. N.D. N.D. -0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.3 N.D. N.D. N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.2 N.D. N.D. N.D. +0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. N.D. N.D. +0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 N.D. N.D. N.D. +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.0 N.D. N.D. N.D. +0.2
auxotrophy YNB Glucose +0.5 N.D. N.D. N.D. -0.2
auxotrophy Dropout Complete +0.4 N.D. N.D. N.D. -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 N.D. N.D. N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. N.D. N.D. +0.3
auxotrophy YNB Glucose 75mM Arginine +0.6 N.D. N.D. N.D. -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 N.D. N.D. N.D. +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 N.D. N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.6 N.D. N.D. N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.6 N.D. N.D. N.D. +0.2
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