Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8466 and 8467 are separated by 934 nucleotides8467 and 8468 are separated by 682 nucleotides8468 and 8469 are separated by 605 nucleotides8469 and 8470 are separated by 663 nucleotides mRNA_98: 8466 - Hypothetical Protein, at 233,874 to 234,659 8466 mRNA_99: 8467 - HMMPfam-Fungal cellulose binding domain-PF00734,HMMPfam-GlcNAc-PI de-N-acetylase-PF02585,ProSitePatterns-CBM1 (carbohydrate binding type-1) domain signature.-PS00562,ProSiteProfiles-Coagulation factors 5/8 type C domain (FA58C) profile.-PS50022,ProSiteProfiles-CBM1 (carbohydrate binding type-1) domain profile.-PS51164,SMART-Fungal-type cellulose-binding domain-SM00236,SUPERFAMILY--SSF102588,SUPERFAMILY--SSF49785,SUPERFAMILY--SSF57180, at 235,594 to 240,654 8467 mRNA_100: 8468 - K03686 dnaJ molecular chaperone DnaJ, at 241,337 to 243,142 8468 mRNA_101: 8469 - K18719 NUP82 nucleoporin NUP82, at 243,748 to 246,867 8469 mRNA_102: 8470 - HMMPfam-PQ loop repeat-PF04193,SMART-Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins.-SM00679, at 247,531 to 248,783 8470
Group Condition mRNA_98 mRNA_99 mRNA_100 mRNA_101 mRNA_102
8466 8467 8468 8469 8470
ypd buoyancy 40 hrs Median Buoyancy -0.2 -0.1 N.D. N.D. -0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 -0.1 N.D. N.D. -0.4
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.2 N.D. N.D. -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 -0.1 N.D. N.D. -0.2
ypd buoyancy 40 hrs High Buoyancy -0.4 -0.0 N.D. N.D. -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 -0.1 N.D. N.D. -0.8
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.1 N.D. N.D. -0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 +0.3 N.D. N.D. -0.4
auxotrophy Dropout Complete -0.0 -0.0 N.D. N.D. -0.4
ypd buoyancy 40 hrs Low Buoyancy -0.3 +0.0 N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.1 N.D. N.D. -0.1
growth on ricinoleic acid YNB Glucose -0.1 +0.1 N.D. N.D. -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 -0.0 N.D. N.D. +0.1
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.0 N.D. N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.1 +0.2 N.D. N.D. -0.0
auxotrophy YNB Glucose +0.1 +0.2 N.D. N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine +0.3 +0.0 N.D. N.D. +0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.3 +0.2 N.D. N.D. -0.1
growth on oleic acid YNB Oleic Acid +0.0 -0.0 N.D. N.D. +0.6
growth on oleic acid YNB Glucose +0.2 -0.3 N.D. N.D. +0.7
growth on ypd YPD +0.5 -0.2 N.D. N.D. +0.5
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