Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8467 and 8468 are separated by 682 nucleotides8468 and 8469 are separated by 605 nucleotides8469 and 8470 are separated by 663 nucleotides8470 and 8471 are separated by 121 nucleotides mRNA_99: 8467 - HMMPfam-Fungal cellulose binding domain-PF00734,HMMPfam-GlcNAc-PI de-N-acetylase-PF02585,ProSitePatterns-CBM1 (carbohydrate binding type-1) domain signature.-PS00562,ProSiteProfiles-Coagulation factors 5/8 type C domain (FA58C) profile.-PS50022,ProSiteProfiles-CBM1 (carbohydrate binding type-1) domain profile.-PS51164,SMART-Fungal-type cellulose-binding domain-SM00236,SUPERFAMILY--SSF102588,SUPERFAMILY--SSF49785,SUPERFAMILY--SSF57180, at 235,594 to 240,654 8467 mRNA_100: 8468 - K03686 dnaJ molecular chaperone DnaJ, at 241,337 to 243,142 8468 mRNA_101: 8469 - K18719 NUP82 nucleoporin NUP82, at 243,748 to 246,867 8469 mRNA_102: 8470 - HMMPfam-PQ loop repeat-PF04193,SMART-Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins.-SM00679, at 247,531 to 248,783 8470 mRNA_103: 8471 - Hypothetical Protein, at 248,905 to 249,863 8471
Group Condition mRNA_99 mRNA_100 mRNA_101 mRNA_102 mRNA_103
8467 8468 8469 8470 8471
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 N.D. N.D. -0.5 -0.5
auxotrophy Dropout Complete -0.0 N.D. N.D. -0.4 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. N.D. -0.8 +0.4
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 N.D. N.D. -0.4 -0.3
growth on ricinoleic acid YNB Glucose +0.1 N.D. N.D. -0.3 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. N.D. -0.5 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. N.D. -0.4 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.0 N.D. N.D. -0.1 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. N.D. +0.0 -0.3
auxotrophy YNB Glucose +0.2 N.D. N.D. -0.1 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 N.D. N.D. +0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. N.D. -0.1 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. N.D. -0.1 +0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. N.D. -0.0 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. N.D. -0.3 +0.6
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. N.D. -0.2 +0.5
growth on ypd YPD -0.2 N.D. N.D. +0.5 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. N.D. -0.2 +0.8
growth on oleic acid YNB Glucose -0.3 N.D. N.D. +0.7 +0.0
growth on oleic acid YNB Oleic Acid -0.0 N.D. N.D. +0.6 -0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.2 N.D. N.D. -0.1 +0.7
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