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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
8470 and 8471 are separated by 121 nucleotides
8471 and 8472 are separated by 363 nucleotides
8472 and 8473 are separated by 15 nucleotides
8473 and 8474 are separated by 377 nucleotides
mRNA_102: 8470 - HMMPfam-PQ loop repeat-PF04193,SMART-Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins.-SM00679, at 247,531 to 248,783
8470
mRNA_103: 8471 - Hypothetical Protein, at 248,905 to 249,863
8471
mRNA_104: 8472 - Hypothetical Protein, at 250,227 to 251,681
8472
mRNA_105: 8473 - K08504 BET1 blocked early in transport 1, at 251,697 to 252,245
8473
mRNA_106: 8474 - K15201 GTP3C3, TFC4 general transcription factor 3C polypeptide 3 (transcription factor C subunit 4), at 252,623 to 256,756
8474
Group
Condition
mRNA
_102
mRNA
_103
mRNA
_104
mRNA
_105
mRNA
_106
8470
8471
8472
8473
8474
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.5
-0.5
-0.1
N.D.
N.D.
ypd buoyancy
40 hrs No Buoyancy Separation
-0.8
+0.4
-0.3
N.D.
N.D.
growth on ricinoleic acid
YNB Glucose
-0.3
-0.1
-0.2
N.D.
N.D.
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.4
-0.3
+0.0
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.4
+0.2
-0.2
N.D.
N.D.
ypd buoyancy
40 hrs Median Buoyancy
-0.5
+0.3
-0.2
N.D.
N.D.
auxotrophy
YNB Glucose 75mM Methionine
+0.0
-0.3
-0.0
N.D.
N.D.
auxotrophy
Dropout Complete
-0.4
-0.3
+0.4
N.D.
N.D.
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.1
-0.1
-0.2
N.D.
N.D.
auxotrophy
YNB Glucose 75mM Arginine
-0.1
-0.2
+0.2
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.3
+0.6
-0.4
N.D.
N.D.
facs on lipid accumulation
Sorted No BODIPY Gate
-0.0
+0.0
-0.1
N.D.
N.D.
auxotrophy
YNB Glucose
-0.1
-0.2
+0.3
N.D.
N.D.
ypd buoyancy
40 hrs Low Buoyancy
-0.1
+0.2
-0.1
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.1
+0.3
-0.3
N.D.
N.D.
growth on ypd
YPD
+0.5
+0.0
-0.3
N.D.
N.D.
ypd buoyancy
40 hrs High Buoyancy
-0.2
+0.5
-0.0
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.2
+0.8
-0.2
N.D.
N.D.
growth on oleic acid
YNB Glucose
+0.7
+0.0
-0.2
N.D.
N.D.
growth on oleic acid
YNB Oleic Acid
+0.6
-0.0
+0.0
N.D.
N.D.
facs on lipid accumulation
Sorted High BODIPY Gate
-0.1
+0.7
+0.1
N.D.
N.D.
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