Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8474 and 8475 are separated by 276 nucleotides8475 and 8476 are separated by 438 nucleotides8476 and 8477 are separated by 108 nucleotides8477 and 8478 are separated by 267 nucleotides mRNA_106: 8474 - K15201 GTP3C3, TFC4 general transcription factor 3C polypeptide 3 (transcription factor C subunit 4), at 252,623 to 256,756 8474 mRNA_107: 8475 - K08592 SENP1 sentrin-specific protease 1, at 257,033 to 257,891 8475 mRNA_108: 8476 - Hypothetical Protein, at 258,330 to 258,956 8476 mRNA_109: 8477 - Hypothetical Protein, at 259,065 to 259,869 8477 mRNA_110: 8478 - KOG3399 Predicted Yippee-type zinc-binding protein, at 260,137 to 260,817 8478
Group Condition mRNA_106 mRNA_107 mRNA_108 mRNA_109 mRNA_110
8474 8475 8476 8477 8478
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. -0.6 -1.8
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. +0.5 -1.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. -0.9 +0.4
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. -0.6 +0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. +0.7 -0.9
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. +0.4 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. +1.5 -1.4
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. +0.2 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. +1.0 -0.3
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. +0.5 +0.4
auxotrophy YNB Glucose N.D. N.D. N.D. +1.3 -0.3
growth on ypd YPD N.D. N.D. N.D. +1.0 +0.0
growth on oleic acid YNB Glucose N.D. N.D. N.D. +0.9 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. +1.2 -0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. +1.4 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. +1.1 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. +1.5 +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. +1.7 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. +1.4 +0.4
auxotrophy Dropout Complete N.D. N.D. N.D. +2.0 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. +1.8 +0.6
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