Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 8481 and 8482 are separated by 348 nucleotides8482 and 8483 are separated by 328 nucleotides8483 and 8484 are separated by 305 nucleotides8484 and 8485 are separated by 555 nucleotides mRNA_113: 8481 - KOG0209 P-type ATPase, at 267,088 to 267,405 8481 mRNA_114: 8482 - K10862 TDP1 tyrosyl-DNA phosphodiesterase 1, at 267,754 to 270,066 8482 mRNA_115: 8483 - K14950 ATP13A1 cation-transporting ATPase 13A1, at 270,395 to 274,744 8483 mRNA_116: 8484 - K06072 DOHH deoxyhypusine monooxygenase, at 275,050 to 276,428 8484 mRNA_117: 8485 - K14815 MRT4 mRNA turnover protein 4, at 276,984 to 278,095 8485
Group Condition mRNA_113 mRNA_114 mRNA_115 mRNA_116 mRNA_117
8481 8482 8483 8484 8485
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -0.2 N.D. N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy -0.7 +0.3 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 -0.2 N.D. N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.3 N.D. N.D. N.D.
growth on ypd YPD -0.4 +0.1 N.D. N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.2 N.D. N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.4 +0.3 N.D. N.D. N.D.
growth on ricinoleic acid YNB Glucose +0.1 +0.0 N.D. N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 +0.1 N.D. N.D. N.D.
growth on oleic acid YNB Glucose +0.0 +0.1 N.D. N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 +0.1 N.D. N.D. N.D.
auxotrophy YNB Glucose +0.2 +0.1 N.D. N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.4 -0.1 N.D. N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 +0.1 N.D. N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.3 N.D. N.D. N.D.
growth on oleic acid YNB Oleic Acid +0.4 +0.2 N.D. N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine +0.6 +0.0 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.7 +0.1 N.D. N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine +0.7 +0.1 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.0 -0.1 N.D. N.D. N.D.
auxotrophy Dropout Complete +1.0 +0.2 N.D. N.D. N.D.
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